BMRB: 11004

NMR Structure of Ebola fusion peptide in SDS micelles at pH 7

Authors

Freitas, M.SANTOS DE., Gaspar, L.PINTO., Lorenzoni, M., Almeida, F.CENEVIVA., Tinoco, L.WANDERLEY., Almeida, M.SILVA., Maia, L.FERNANDES., Degreve, L., Valente, A., Silva, J.LIMA.

Assembly

Ebola fusion peptide

Entity

1. Ebola fusion peptide (polymer, Thiol state: not present), 16 monomers, 1574.819 Da Detail

GAAIGLAWIP YFGPAA

Formula weight

1574.819 Da

Source organism

Ebola virus sp.

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 87.2 %, Completeness (bb): 93.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	87.2 % (75 of 86)	87.2 % (75 of 86)
Backbone	93.9 % (31 of 33)	93.9 % (31 of 33)
Sidechain	83.0 % (44 of 53)	83.0 % (44 of 53)
Aromatic	60.0 % (9 of 15)	60.0 % (9 of 15)
Methyl	90.9 % (10 of 11)	90.9 % (10 of 11)

1. fusion peptide

GAAIGLAWIP YFGPAA

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	Ebola fusion peptide	natural abundance		2 mM
2	SDS	natural abundance		400 mM
3	potassium phosphate	natural abundance		15 mM
4	D2O			10 %
5	H2O			90 %

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Calculated from 20 models in PDB: 2RLJ, Strand ID: A Detail

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Release date

2008-06-24

Citation

Structure of the Ebola fusion peptide in a membrane-mimetic environment and the interaction with lipid rafts
Freitas, M.SANTOS DE., Gaspar, L.PINTO., Lorenzoni, M., Almeida, F.CENEVIVA., Tinoco, L.WANDERLEY., Almeida, M.SILVA., Maia, L.FERNANDES., Degreve, L., Valente, A., Silva, J.LIMA.
J. Biol. Chem. (2007), 282, 27306-27314, PubMed 17545161 , DOI 10.1074/jbc.M611864200 , Abstract

Related entities 1. Ebola fusion peptide, : 1 : 22 : 2 : 2 : 12 entities Detail

Interaction partners 1. Ebola fusion peptide, : 1 interactors Detail

Experiments performed 2 experiments Detail