BMRB: 11007

FBP28WW2 domain in complex with PTPPPLPP peptide

Authors

Ramirez-Espain, X., Ruiz, L., Martin-Malpartida, P., Oschkinat, H., Macias, M.J.

Assembly

FBP28WW2-PTPPPLPP

Entity

1. FBP28WW2 (polymer, Thiol state: not present), 37 monomers, 4359.668 Da Detail

GATAVSEWTE YKTADGKTYY YNNRTLESTW EKPQELK

2. PTPPPLPP (polymer, Thiol state: not present), 8 monomers, 814.9650 Da Detail

PTPPPLPP

Total weight

5174.633 Da

Max. entity weight

4359.668 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 86.5 %, Completeness (bb): 95.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	86.5 % (243 of 281)	86.5 % (243 of 281)
Backbone	95.3 % (81 of 85)	95.3 % (81 of 85)
Sidechain	82.7 % (162 of 196)	82.7 % (162 of 196)
Aromatic	100.0 % (28 of 28)	100.0 % (28 of 28)
Methyl	94.4 % (17 of 18)	94.4 % (17 of 18)

1. FBP28WW2

GATAVSEWTE YKTADGKTYY YNNRTLESTW EKPQELK

2. PTPPPLPP

PTPPPLPP

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 285 K, pH 5.8

#	Name	Isotope labeling	Type	Concentration
1	entity_1	natural abundance		1 mM
2	entity_2	natural abundance		3 mM
3	sodium phosphate	natural abundance		100 mM
4	sodium chloride	natural abundance		50 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 285 K, pH 5.8

#	Name	Isotope labeling	Type	Concentration
5	entity_1	natural abundance		1 mM
6	entity_2	natural abundance		3 mM
7	sodium phosphate	natural abundance		100 mM
8	sodium chloride	natural abundance		50 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 285 K, pH 5.8

#	Name	Isotope labeling	Type	Concentration
9	entity_1	[U-100% 15N]		1 mM
10	entity_2	natural abundance		3 mM
11	sodium phosphate	natural abundance		100 mM
12	sodium chloride	natural abundance		50 mM

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Protein Blocks Logo

Calculated from 8 models in PDB: 2RLY, Strand ID: P, W Detail

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Release date

2008-06-24

Citation

Structural characterization of a new binding motif and a novel binding mode in group 2 WW domains
Ramirez-Espain, X., Ruiz, L., Martin-Malpartida, P., Oschkinat, H., Macias, M.J.
J. Mol. Biol. (2007), 373, 1255-1268, PubMed 17915251 , DOI 10.1016/j.jmb.2007.08.052 , Abstract

Related entities 1. FBP28WW2, : 5 : 104 entities Detail

Related entities 2. PTPPPLPP, : 2 entities Detail

Interaction partners 1. FBP28WW2, : 20 interactors Detail

Experiments performed 7 experiments Detail