BMRB: 11008

FBP28WW2 domain in complex with a PPPLIPPPP peptide

Authors

Ramirez-Espain, X., Ruiz, L., Martin-Malpartida, P., Oschkinat, H., Macias, M.J.

Assembly

FBP28WW2-PPPLIPPPP

Entity

1. FBP28WW2 (polymer, Thiol state: not present), 37 monomers, 4359.668 Da Detail

GATAVSEWTE YKTADGKTYY YNNRTLESTW EKPQELK

2. PPPLIPPPP (polymer, Thiol state: not present), 9 monomers, 924.1338 Da Detail

PPPLIPPPP

Total weight

5283.802 Da

Max. entity weight

4359.668 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 85.2 %, Completeness (bb): 97.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	85.2 % (248 of 291)	85.2 % (248 of 291)
Backbone	97.7 % (84 of 86)	97.7 % (84 of 86)
Sidechain	80.0 % (164 of 205)	80.0 % (164 of 205)
Aromatic	100.0 % (28 of 28)	100.0 % (28 of 28)
Methyl	94.7 % (18 of 19)	94.7 % (18 of 19)

1. FBP28WW2

GATAVSEWTE YKTADGKTYY YNNRTLESTW EKPQELK

2. PPPLIPPPP

PPPLIPPPP

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 285 K, pH 5.8

#	Name	Isotope labeling	Type	Concentration
1	FPB28WW2	natural abundance		1 mM
2	PPPLIPPPP	natural abundance		3 mM
3	sodium phosphate	natural abundance		50 mM
4	sodium chloride	natural abundance		100 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 285 K, pH 5.8

#	Name	Isotope labeling	Type	Concentration
5	FPB28WW2	natural abundance		1 mM
6	PPPLIPPPP	natural abundance		3 mM
7	sodium phosphate	natural abundance		50 mM
8	sodium chloride	natural abundance		100 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 285 K, pH 5.8

#	Name	Isotope labeling	Type	Concentration
9	FPB28WW2	[U-100% 15N]		1 mM
10	PPPLIPPPP	natural abundance		3 mM
11	sodium phosphate	natural abundance		50 mM
12	sodium chloride	natural abundance		100 mM

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Protein Blocks Logo

Calculated from 8 models in PDB: 2RM0, Strand ID: P, W Detail

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Release date

2008-06-24

Citation

Structural characterization of a new binding motif and a novel binding mode in group 2 WW domains
Ramirez-Espain, X., Ruiz, L., Martin-Malpartida, P., Oschkinat, H., Macias, M.J.
J. Mol. Biol. (2007), 373, 1255-1268, PubMed 17915251 , DOI 10.1016/j.jmb.2007.08.052 , Abstract

Entries sharing articles BMRB: 2 entries Detail

Related entities 1. FBP28WW2, : 5 : 104 entities Detail

Experiments performed 6 experiments Detail