BMRBj - BMREntryMaster

Found 1 Document (1.058 seconds)

BMRB: 11009

2RM4

SOLUTION STRUCTURE OF THE LSM DOMAIN OF Dm EDC3 (ENHANCER OF DECAPPING 3)

Authors

Truffault, V., Coles, M., Tritschler, F.

Assembly

Dm LSm16 monomer

Entity

1. Dm LSm16 monomer (polymer, Thiol state: all free), 103 monomers, 11083.70 Da Detail

GAMGPTDQDW IGCAVSIACD EVLGVFQGLI KQISAEEITI VRAFRNGVPL RKQNAEVVLK CTDIRSIDLI EPAKQDLDGH TAPPPVVNKP TPVKLPHFSN ILG

Formula weight

11083.7 Da

Source organism

Drosophila melanogaster

Exptl. method

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.1 %, Completeness: 86.9 %, Completeness (bb): 92.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.9 % (1019 of 1173)	86.5 % (527 of 609)	86.5 % (398 of 460)	90.4 % (94 of 104)
Backbone	92.0 % (552 of 600)	91.7 % (188 of 205)	92.0 % (277 of 301)	92.6 % (87 of 94)
Sidechain	83.4 % (557 of 668)	83.9 % (339 of 404)	83.1 % (211 of 254)	70.0 % (7 of 10)
Aromatic	34.0 % (17 of 50)	64.0 % (16 of 25)	0.0 % (0 of 24)	100.0 % (1 of 1)
Methyl	95.7 % (132 of 138)	95.7 % (66 of 69)	95.7 % (66 of 69)

1. Dm EDC3

GAMGPTDQDW IGCAVSIACD EVLGVFQGLI KQISAEEITI VRAFRNGVPL RKQNAEVVLK CTDIRSIDLI EPAKQDLDGH TAPPPVVNKP TPVKLPHFSN ILG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.1

#	Name	Isotope labeling	Type	Concentration
1	Dm EDC3	[U-100% 15N]		1.2 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.1

#	Name	Isotope labeling	Type	Concentration
2	Dm EDC3	[U-100% 13C; U-100% 15N]		0.8 mM

LACS Plot; CA

Referencing offset: -0.02 ppm, Outliers: 5 Detail

LACS Plot; CB

Referencing offset: -0.02 ppm, Outliers: 5 Detail

LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 3 Detail

LACS Plot; CO

Referencing offset: 0.41 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 22 models in PDB: 2RM4, Strand ID: A Detail

Release date

2008-06-24

Citation

A divergent Sm fold in EDC3 proteins mediates DCP1 binding and P-body targeting
Tritschler, F., Eulalio, A., Truffault, V., Hartmann, M.D., Helms, S., Schmidt, S., Coles, M., Izaurralde, E., Weichenrieder, O.
Mol. Cell. Biol. (2007), 27, 8600-8611, PubMed 17923697 , DOI 10.1128/MCB.01506-07 , Abstract

Related entities 1. Dm LSm16 monomer, : 1 : 2 : 1 entities Detail

Interaction partners 1. Dm LSm16 monomer, : 3 interactors Detail

More details for 3 interactors identified by 3 citations

Experiments performed 17 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.1

#	Name	Isotope labeling	Type	Concentration
1	Dm EDC3	[U-100% 15N]		1.2 mM

2 2D 1H-15N HSQC

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.1

#	Name	Isotope labeling	Type	Concentration
2	Dm EDC3	[U-100% 13C; U-100% 15N]		0.8 mM

3 2D 1H-13C HSQC

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.1

#	Name	Isotope labeling	Type	Concentration
2	Dm EDC3	[U-100% 13C; U-100% 15N]		0.8 mM

4 3D CBCA(CO)NH

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.1

#	Name	Isotope labeling	Type	Concentration
2	Dm EDC3	[U-100% 13C; U-100% 15N]		0.8 mM

5 3D C(CO)NH

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.1

#	Name	Isotope labeling	Type	Concentration
2	Dm EDC3	[U-100% 13C; U-100% 15N]		0.8 mM

6 3D CCH-COSY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.1

#	Name	Isotope labeling	Type	Concentration
2	Dm EDC3	[U-100% 13C; U-100% 15N]		0.8 mM

7 3D CCH-TOCSY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.1

#	Name	Isotope labeling	Type	Concentration
2	Dm EDC3	[U-100% 13C; U-100% 15N]		0.8 mM

8 3D CNH-NOESY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.1

#	Name	Isotope labeling	Type	Concentration
2	Dm EDC3	[U-100% 13C; U-100% 15N]		0.8 mM

9 3D 1H-13C NOESY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.1

#	Name	Isotope labeling	Type	Concentration
2	Dm EDC3	[U-100% 13C; U-100% 15N]		0.8 mM

10 3D HNCA

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.1

#	Name	Isotope labeling	Type	Concentration
2	Dm EDC3	[U-100% 13C; U-100% 15N]		0.8 mM

11 3D HN(CA)CO

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.1

#	Name	Isotope labeling	Type	Concentration
2	Dm EDC3	[U-100% 13C; U-100% 15N]		0.8 mM

12 3D HNCO

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.1

#	Name	Isotope labeling	Type	Concentration
2	Dm EDC3	[U-100% 13C; U-100% 15N]		0.8 mM

13 3D HNHA

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.1

#	Name	Isotope labeling	Type	Concentration
1	Dm EDC3	[U-100% 15N]		1.2 mM

14 3D HNHB

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.1

#	Name	Isotope labeling	Type	Concentration
1	Dm EDC3	[U-100% 15N]		1.2 mM

15 3D 1H-15N NOESY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.1

#	Name	Isotope labeling	Type	Concentration
1	Dm EDC3	[U-100% 15N]		1.2 mM

16 3D NNH-NOESY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.1

#	Name	Isotope labeling	Type	Concentration
1	Dm EDC3	[U-100% 15N]		1.2 mM

17 2D 1H-1H NOESY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.1

#	Name	Isotope labeling	Type	Concentration
1	Dm EDC3	[U-100% 15N]		1.2 mM

nullKeywords ENHANCER OF MRNA DECAPPING, P-BODY COMPONENT, SM-LIKE PROTEIN