BMRB: 11010

Rac1/PRK1 Complex

Authors

Modha, R., Campbell, L.J., Nietlispach, D., Buhecha, H.R., Owen, D., Mott, H.R.

Assembly

Rac1/HR1b complex

Entity

1. Rac1 (polymer, Thiol state: all free), 192 monomers, 21434.88 Da Detail

MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDGKP VNLGLWDTAG LEDYDRLRPL SYPQTDVFLI CFSLVSPASF ENVRAKWYPE VRHHCPNTPI ILVGTKLDLR DDKDTIEKLK EKKLTPITYP QGLAMAKEIG AVKYLECSAL TQRGLKTVFD EAIRAVLCPP PVKKRKRKCL LL

2. PRK1 HR1b (polymer, Thiol state: not present), 81 monomers, 9005.319 Da Detail

GIPATNLSRV AGLEKQLAIE LKVKQGAENM IQTYSNGSTK DRKLLLTAQQ MLQDSKTKID IIRMQLRRAL QADQLENQAA P

3. GCP (non-polymer), 521.208 Da

Total weight

30961.406 Da

Max. entity weight

21434.88 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.4 %, Completeness: 82.1 %, Completeness (bb): 85.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	82.1 % (2650 of 3226)	82.0 % (1382 of 1685)	80.2 % (1009 of 1258)	91.5 % (259 of 283)
Backbone	85.0 % (1365 of 1606)	89.0 % (485 of 545)	80.2 % (645 of 804)	91.4 % (235 of 257)
Sidechain	80.9 % (1519 of 1878)	78.7 % (897 of 1140)	84.0 % (598 of 712)	92.3 % (24 of 26)
Aromatic	48.2 % (79 of 164)	52.4 % (43 of 82)	42.5 % (34 of 80)	100.0 % (2 of 2)
Methyl	92.9 % (327 of 352)	93.2 % (164 of 176)	92.6 % (163 of 176)

1. Rac1

MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDGKP VNLGLWDTAG LEDYDRLRPL SYPQTDVFLI CFSLVSPASF ENVRAKWYPE VRHHCPNTPI ILVGTKLDLR DDKDTIEKLK EKKLTPITYP QGLAMAKEIG AVKYLECSAL TQRGLKTVFD EAIRAVLCPP PVKKRKRKCL LL

2. PRK1 HR1b

GIPATNLSRV AGLEKQLAIE LKVKQGAENM IQTYSNGSTK DRKLLLTAQQ MLQDSKTKID IIRMQLRRAL QADQLENQAA P

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
11	Rac1	[U-100% 13C; U-100% 15N]		0.6 mM
12	PRK1 HR1b	natural abundance		0.6 mM
13	PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER	natural abundance		0.7 mM
14	Tris-HCl	natural abundance		10 mM
15	NaCl	natural abundance		200 mM
16	MgCl2	natural abundance		5 mM
17	dithiothreitol	natural abundance		10 mM
18	NaN3	natural abundance		0.05 %
19	H2O	natural abundance		90 %
20	D2O			10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
31	Rac1	natural abundance		0.6 mM
32	PRK1 HR1b	[U-100% 13C; U-100% 15N]		0.6 mM
33	PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER	natural abundance		0.7 mM
34	Tris-HCl	natural abundance		10 mM
35	NaCl	natural abundance		200 mM
36	MgCl2	natural abundance		5 mM
37	dithiothreitol	natural abundance		10 mM
38	NaN3	natural abundance		0.05 %
39	H2O	natural abundance		90 %
40	D2O			10 %

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Calculated from 25 models in PDB: 2RMK, Strand ID: A, B Detail

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Release date

2008-06-26

Citation

The Rac1 polybasic region is required for interaction with its effector PRK1
Modha, R., Campbell, L.J., Nietlispach, D., Buhecha, H.R., Owen, D., Mott, H.R.
J. Biol. Chem. (2008), 283, 1492-1500, PubMed 18006505 , DOI 10.1074/jbc.M706760200 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Rac1, : 4 : 97 entities Detail

Related entities 2. PRK1 HR1b, : 1 : 1 : 2 : 30 entities Detail

Interaction partners 2. PRK1 HR1b, : 27 interactors Detail

Experiments performed 2 experiments Detail