BMRB: 11011

Solution structure of the N-terminal soluble domains of Bacillus subtilis CopA

Authors

Singleton, C., Banci, L., Bertini, I., Ciofi-Baffoni, S., Tenori, L., Kihlken, M.A., Boetzel, R., Le Brun, N.E.

Assembly

CopA

Entity

1. CopA (polymer, Thiol state: all free), 147 monomers, 15913.00 Da Detail

MLSEQKEIAM QVSGMTCAAC AARIEKGLKR MPGVTDANVN LATETSNVIY DPAETGTAAI QEKIEKLGYH VVTEKAEFDI EGMTCAACAN RIEKRLNKIE GVANAPVNFA LETVTVEYNP KEASVSDLKE AVDKLGYKLK LKGEQDS

Formula weight

15913.0 Da

Source organism

Bacillus subtilis

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.6 %, Completeness: 83.6 %, Completeness (bb): 83.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	83.6 % (1373 of 1643)	90.6 % (771 of 851)	71.0 % (452 of 637)	96.8 % (150 of 155)
Backbone	83.1 % (726 of 874)	96.3 % (288 of 299)	69.2 % (299 of 432)	97.2 % (139 of 143)
Sidechain	83.0 % (753 of 907)	84.2 % (465 of 552)	80.8 % (277 of 343)	91.7 % (11 of 12)
Aromatic	8.9 % (5 of 56)	17.9 % (5 of 28)	0.0 % (0 of 28)
Methyl	86.5 % (154 of 178)	80.9 % (72 of 89)	92.1 % (82 of 89)

1. CopA

MLSEQKEIAM QVSGMTCAAC AARIEKGLKR MPGVTDANVN LATETSNVIY DPAETGTAAI QEKIEKLGYH VVTEKAEFDI EGMTCAACAN RIEKRLNKIE GVANAPVNFA LETVTVEYNP KEASVSDLKE AVDKLGYKLK LKGEQDS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		1 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

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LACS Plot; CA

Referencing offset: 2.59 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	dioxane	methylene carbons	0.0 ppm	internal	direct	1.0
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 2.59 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	dioxane	methylene carbons	0.0 ppm	internal	direct	1.0
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	dioxane	methylene carbons	0.0 ppm	internal	direct	1.0
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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Protein Blocks Logo

Calculated from 25 models in PDB: 2RML, Strand ID: A Detail

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Release date

2008-06-26

Citation

Structure and Cu(I)-binding properties of the N-terminal soluble domains of Bacillus subtilis CopA
Singleton, C., Banci, L., Ciofi-Baffoni, S., Tenori, L., Kihlken, M.A., Boetzel, R., Le Brun, N.E.
Biochem. J. (2008), 411, 571-579, PubMed 18215122 , DOI 10.1042/BJ20071620 , Abstract

Related entities 1. CopA, : 1 : 1 : 163 entities Detail

Interaction partners 1. CopA, : 2 interactors Detail

Experiments performed 9 experiments Detail