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BMRB: 11019

2RN9

Cox17

Authors

Banci, L., Bertini, I., Ciofi-Baffoni, S., Janicka, A., Martinelli, M., Kozlowski, H., Palumaa, P.

Assembly

Cox17 monomer

Entity

1. Cox17 monomer (polymer, Thiol state: free and disulfide bound), 67 monomers, 7307.450 Da Detail

GSFTMPGLVD SNPAPPESQE KKPLKPCCAC PETKKARDAC IIEKGEEHCG HLIEAHKECM RALGFKI

Formula weight

7307.45 Da

Entity Connection

disulfide 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS30:SG	1:CYS59:SG
2	disulfide	sing	1:CYS40:SG	1:CYS49:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.5 %, Completeness: 88.6 %, Completeness (bb): 90.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.6 % (675 of 762)	90.6 % (367 of 405)	85.4 % (252 of 295)	90.3 % (56 of 62)
Backbone	90.7 % (352 of 388)	90.9 % (120 of 132)	90.8 % (178 of 196)	90.0 % (54 of 60)
Sidechain	87.4 % (381 of 436)	90.5 % (247 of 273)	82.0 % (132 of 161)	100.0 % (2 of 2)
Aromatic	34.4 % (11 of 32)	68.8 % (11 of 16)	0.0 % (0 of 16)
Methyl	100.0 % (52 of 52)	100.0 % (26 of 26)	100.0 % (26 of 26)

1. Cox17

GSFTMPGLVD SNPAPPESQE KKPLKPCCAC PETKKARDAC IIEKGEEHCG HLIEAHKECM RALGFKI

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.2) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Cox17	natural abundance		0.5 ~ 1.0 mM
2	DTT	natural abundance		1 mM
3	potassium phosphate	natural abundance		50 mM
4	H2O		solvent	90 %
5	D2O		solvent	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.2) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	Cox17	[U-100% 15N]		0.5 ~ 1.0 mM
7	DTT	natural abundance		1 mM
8	potassium phosphate	natural abundance		50 mM
9	H2O		solvent	90 %
10	D2O		solvent	10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.2) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Type	Concentration
11	Cox17	[U-100% 13C; U-100% 15N]		0.5 ~ 1.0 mM
12	DTT	natural abundance		1 mM
13	potassium phosphate	natural abundance		50 mM
14	H2O		solvent	90 %
15	D2O		solvent	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.87 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.87 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.12 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.79 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2RN9, Strand ID: A Detail

Release date

2008-06-26

Citation

A structural-dynamical characterization of human Cox17
Banci, L., Bertini, I., Ciofi Baffoni, S., Janicka, A., Martinelli, M., Kozlowski, H., Palumaa, P.
J. Biol. Chem. (2008), 283, 7912-7920, PubMed 18093982 , DOI 10.1074/jbc.M708016200 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

  BMRB: 11020 released on 2008-01-08
    Title Solution structure of human Cu(I)Cox17
  Swiss-Prot: Q14061 released on 1997-11-01
    Title COX17_HUMAN Entity Cytochrome c oxidase copper chaperone

Related entities 1. Cox17 monomer, : 1 : 1 : 3 : 17 entities Detail

Interaction partners 1. Cox17 monomer, : 15 interactors Detail

More details for 15 interactors identified by 5 citations

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC