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BMRB: 11020

2RNB

Solution structure of human Cu(I)Cox17

Authors

Banci, L., Bertini, I., Ciofi Baffoni, S., Janicka, A., Martinelli, M., Kozlowski, H., Palumaa, P.

Assembly

Cu(I)Cox17

Entity

1. Cox17 (polymer, Thiol state: free and disulfide bound), 67 monomers, 7307.450 Da Detail

GSFTMPGLVD SNPAPPESQE KKPLKPCCAC PETKKARDAC IIEKGEEHCG HLIEAHKECM RALGFKI

2. CU1 (non-polymer), 63.546 Da

Total weight

7370.996 Da

Max. entity weight

7307.45 Da

Entity Connection

disulfide 2, metal coordination 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	metal coordination	sing	1:CYS27:SG	2:CU11:CU
2	metal coordination	sing	1:CYS28:SG	2:CU11:CU
3	disulfide	sing	1:CYS30:SG	1:CYS59:SG
4	disulfide	sing	1:CYS40:SG	1:CYS49:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.0 %, Completeness: 85.0 %, Completeness (bb): 79.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.0 % (648 of 762)	94.3 % (382 of 405)	70.8 % (209 of 295)	91.9 % (57 of 62)
Backbone	79.1 % (307 of 388)	93.2 % (123 of 132)	65.8 % (129 of 196)	91.7 % (55 of 60)
Sidechain	92.0 % (401 of 436)	94.9 % (259 of 273)	87.0 % (140 of 161)	100.0 % (2 of 2)
Aromatic	34.4 % (11 of 32)	68.8 % (11 of 16)	0.0 % (0 of 16)
Methyl	96.2 % (50 of 52)	92.3 % (24 of 26)	100.0 % (26 of 26)

1. Cox17

GSFTMPGLVD SNPAPPESQE KKPLKPCCAC PETKKARDAC IIEKGEEHCG HLIEAHKECM RALGFKI

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.2) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	Cox17	natural abundance	0.5 ~ 1.0 mM
2	COPPER (I) ION	natural abundance	0.5 ~ 1.0 mM
3	DTT	natural abundance	1 mM
4	potassium phosphate	natural abundance	50 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.2) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	Cox17	[U-100% 15N]	0.5 ~ 1.0 mM
8	COPPER (I) ION	natural abundance	0.5 ~ 1.0 mM
9	DTT	natural abundance	1 mM
10	potassium phosphate	natural abundance	50 mM
11	H2O		90 %
12	D2O		10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.2) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
13	Cox17	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 mM
14	COPPER (I) ION	natural abundance	0.5 ~ 1.0 mM
15	DTT	natural abundance	1 mM
16	potassium phosphate	natural abundance	50 mM
17	H2O		90 %
18	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.74 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.74 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.12 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2RNB, Strand ID: A Detail

Release date

2008-01-08

Citation

A structural-dynamical characterization of human Cox17
Banci, L., Bertini, I., Ciofi Baffoni, S., Janicka, A., Martinelli, M., Kozlowski, H., Palumaa, P.
J. Biol. Chem. (2008), 283, 7912-7920, PubMed 18093982 , DOI 10.1074/jbc.M708016200 , Abstract

Related entities 1. Cox17, : 1 : 1 : 3 : 17 entities Detail

Interaction partners 1. Cox17, : 15 interactors Detail

More details for 15 interactors identified by 5 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC