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BMRB: 11029

2RNN

Solution Structure of the N-terminal SAP Domain of SUMO E3 Ligases from Saccharomyces cerevisiae

Authors

Suzuki, R., Shindo, H., Tase, A., Yamazaki, T.

Assembly

Siz1 1-111

Entity

1. Siz1 1-111 (polymer, Thiol state: all free), 114 monomers, 13224.00 Da Detail

GSHMINLEDY WEDETPGPDR EPTNELRNEV EETITLMELL KVSELKDICR SVSFPVSGRK AVLQDLIRNF LQNALVVGKS DPYRVQAVKF LIERIRKNEP LPVYKDLWNA LRKG

Formula weight

13224.0 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.9 %, Completeness (bb): 98.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.9 % (1312 of 1382)	95.7 % (697 of 728)	93.3 % (499 of 535)	97.5 % (116 of 119)
Backbone	98.1 % (657 of 670)	98.7 % (223 of 226)	97.6 % (329 of 337)	98.1 % (105 of 107)
Sidechain	92.9 % (763 of 821)	94.4 % (474 of 502)	90.6 % (278 of 307)	91.7 % (11 of 12)
Aromatic	72.0 % (59 of 82)	85.4 % (35 of 41)	59.0 % (23 of 39)	50.0 % (1 of 2)
Methyl	97.1 % (132 of 136)	100.0 % (68 of 68)	94.1 % (64 of 68)

1. Siz1 1-111

GSHMINLEDY WEDETPGPDR EPTNELRNEV EETITLMELL KVSELKDICR SVSFPVSGRK AVLQDLIRNF LQNALVVGKS DPYRVQAVKF LIERIRKNEP LPVYKDLWNA LRKG

Show sample condition

Sample #1

Solvent system 92% H2O/8% D2O, Pressure 1 atm, Temperature 283 K, pH 6.1

#	Name	Isotope labeling	Concentration
1	Siz1 1-111	[U-15N]	0.55 mM
2	potassium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	300 mM
4	DTT	natural abundance	3 mM
5	H2O	natural abundance	92 %
6	D2O		8 %

Sample #2

Solvent system 92% H2O/8% D2O, Pressure 1 atm, Temperature 283 K, pH 6.1

#	Name	Isotope labeling	Concentration
7	Siz1 1-111	[U-13C; U-15N]	0.6 mM
8	potassium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	300 mM
10	DTT	natural abundance	3 mM
11	H2O	natural abundance	92 %
12	D2O		8 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 283 K, pH 6.1

#	Name	Isotope labeling	Concentration
13	Siz1 1-111	[U-13C; U-15N]	0.69 mM
14	potassium phosphate	natural abundance	20 mM
15	sodium chloride	natural abundance	300 mM
16	DTT	natural abundance	3 mM
17	D2O		100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbons	external	direct	1.0
¹H	TSP	methyl protons	external	indirect	1.0
¹⁵N	ammonium hydroxide	nitrogen	external	direct	1.0

Referencing offset: 0.56 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbons	external	direct	1.0
¹H	TSP	methyl protons	external	indirect	1.0
¹⁵N	ammonium hydroxide	nitrogen	external	direct	1.0

Referencing offset: 0.56 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbons	external	direct	1.0
¹H	TSP	methyl protons	external	indirect	1.0
¹⁵N	ammonium hydroxide	nitrogen	external	direct	1.0

Referencing offset: 0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbons	external	direct	1.0
¹H	TSP	methyl protons	external	indirect	1.0
¹⁵N	ammonium hydroxide	nitrogen	external	direct	1.0

Referencing offset: 0.16 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2RNN, Strand ID: A Detail

Release date

2008-11-10

Citation

Solution structures and DNA binding properties of the N-terminal SAP domains of SUMO E3 ligases from Saccharomyces cerevisiae and Oryza sativa
Suzuki, R., Shindo, H., Tase, A., Kikuchi, Y., Shimizu, M., Yamazaki, T.
Proteins (2009), 75, 336-347, PubMed 18831036 , DOI 10.1002/prot.22243 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

  BMRB: 11030 released on 2008-11-10
    Title Solution Structure of the N-terminal SAP Domain of SUMO E3 Ligases from Oryza sativa
  Swiss-Prot: Q6L4L4 released on 2009-09-22
    Title SIZ1_ORYSJ Entity E3 SUMO-protein ligase SIZ1

Related entities 1. Siz1 1-111, : 1 : 2 : 6 entities Detail

Interaction partners 1. Siz1 1-111, : 10 interactors Detail

More details for 10 interactors identified by 4 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC