BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	76.2 % (1095 of 1437)	74.8 % (560 of 749)	74.8 % (421 of 563)	91.2 % (114 of 125)
Backbone	95.1 % (681 of 716)	93.4 % (226 of 242)	95.6 % (344 of 360)	97.4 % (111 of 114)
Sidechain	63.1 % (528 of 837)	65.9 % (334 of 507)	59.9 % (191 of 319)	27.3 % (3 of 11)
Aromatic	16.9 % (20 of 118)	28.8 % (17 of 59)	0.0 % (0 of 56)	100.0 % (3 of 3)
Methyl	83.8 % (124 of 148)	89.2 % (66 of 74)	78.4 % (58 of 74)

1. The DNA binding domain of RTBP1

GSPFADPNSL ALANVPLSRS KRPDFGQRRI RRPFTVAEVE LLVEAVEHLG TGRWRDVKFR AFENVHHRTY VDLKDKWKTL VHTASIAPQQ RRGAPVPQEL LDRVLAAQAY WSVDSSGRIV TL

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	The DNA binding domai of RTBP1	[U-99% 15N]	1 mM
2	Potassium phosphate	natural abundance	50 mM
3	NaCl	natural abundance	100 mM
4	NaN3	natural abundance	1 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
12	The DNA binding domai of RTBP1	[U-99% 13C; U-99% 15N]	1 mM
13	Potassium phosphate	natural abundance	50 mM
14	NaCl	natural abundance	100 mM
15	NaN3	natural abundance	1 mM
16	H2O		90 %
17	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.12 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.12 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.28 ppm, Outliers: 5 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2ROH, Strand ID: A Detail

Release date

2009-04-28

Citation

Solution structure of the DNA binding domain of rice telomere binding protein RTBP1
Ko, S., Yu, E., Shin, J., Yoo, H., Tanaka, T., Kim, W., Cho, H., Lee, W., Chung, I.
Biochemistry (2009), 48, 827-838, PubMed 19152316 , DOI 10.1021/bi801270g , Abstract

Related entities 1. The DNA binding domain of RTBP1, : 1 : 2 : 21 entities Detail

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DRX - 900 MHz cryo-probe system installed

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	The DNA binding domai of RTBP1	[U-99% 15N]	1 mM
2	Potassium phosphate	natural abundance	50 mM
3	NaCl	natural abundance	100 mM
4	NaN3	natural abundance	1 mM
5	H2O		90 %
6	D2O		10 %

2 2D 1H-15N HSQC

Instrument

Bruker DRX - 500 MHz cryo-probe system installed

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	The DNA binding domai of RTBP1	[U-99% 15N]	1 mM
2	Potassium phosphate	natural abundance	50 mM
3	NaCl	natural abundance	100 mM
4	NaN3	natural abundance	1 mM
5	H2O		90 %
6	D2O		10 %

3 2D 1H-13C HSQC

Instrument

Bruker DRX - 500 MHz cryo-probe system installed

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
12	The DNA binding domai of RTBP1	[U-99% 13C; U-99% 15N]	1 mM
13	Potassium phosphate	natural abundance	50 mM
14	NaCl	natural abundance	100 mM
15	NaN3	natural abundance	1 mM
16	H2O		90 %
17	D2O		10 %

4 3D CBCA(CO)NH

Instrument

Bruker DRX - 500 MHz cryo-probe system installed

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
12	The DNA binding domai of RTBP1	[U-99% 13C; U-99% 15N]	1 mM
13	Potassium phosphate	natural abundance	50 mM
14	NaCl	natural abundance	100 mM
15	NaN3	natural abundance	1 mM
16	H2O		90 %
17	D2O		10 %

5 3D HNCO

Instrument

Bruker DRX - 500 MHz cryo-probe system installed

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
12	The DNA binding domai of RTBP1	[U-99% 13C; U-99% 15N]	1 mM
13	Potassium phosphate	natural abundance	50 mM
14	NaCl	natural abundance	100 mM
15	NaN3	natural abundance	1 mM
16	H2O		90 %
17	D2O		10 %

6 3D HNCA

Instrument

Bruker DRX - 500 MHz cryo-probe system installed

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
12	The DNA binding domai of RTBP1	[U-99% 13C; U-99% 15N]	1 mM
13	Potassium phosphate	natural abundance	50 mM
14	NaCl	natural abundance	100 mM
15	NaN3	natural abundance	1 mM
16	H2O		90 %
17	D2O		10 %

7 3D HNCACB

Instrument

Bruker DRX - 500 MHz cryo-probe system installed

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
12	The DNA binding domai of RTBP1	[U-99% 13C; U-99% 15N]	1 mM
13	Potassium phosphate	natural abundance	50 mM
14	NaCl	natural abundance	100 mM
15	NaN3	natural abundance	1 mM
16	H2O		90 %
17	D2O		10 %

8 3D HBHA(CO)NH

Instrument

Bruker DRX - 500 MHz cryo-probe system installed

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
12	The DNA binding domai of RTBP1	[U-99% 13C; U-99% 15N]	1 mM
13	Potassium phosphate	natural abundance	50 mM
14	NaCl	natural abundance	100 mM
15	NaN3	natural abundance	1 mM
16	H2O		90 %
17	D2O		10 %

9 3D HCCH-TOCSY

Instrument

Bruker DRX - 500 MHz cryo-probe system installed

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
12	The DNA binding domai of RTBP1	[U-99% 13C; U-99% 15N]	1 mM
13	Potassium phosphate	natural abundance	50 mM
14	NaCl	natural abundance	100 mM
15	NaN3	natural abundance	1 mM
16	H2O		90 %
17	D2O		10 %

10 3D 1H-15N NOESY

Instrument

Bruker DRX - 500 MHz cryo-probe system installed

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
12	The DNA binding domai of RTBP1	[U-99% 13C; U-99% 15N]	1 mM
13	Potassium phosphate	natural abundance	50 mM
14	NaCl	natural abundance	100 mM
15	NaN3	natural abundance	1 mM
16	H2O		90 %
17	D2O		10 %

11 3D 1H-13C NOESY

Instrument

Bruker DRX - 500 MHz cryo-probe system installed

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
12	The DNA binding domai of RTBP1	[U-99% 13C; U-99% 15N]	1 mM
13	Potassium phosphate	natural abundance	50 mM
14	NaCl	natural abundance	100 mM
15	NaN3	natural abundance	1 mM
16	H2O		90 %
17	D2O		10 %

12 3D HNHA

Instrument

Bruker DRX - 500 MHz cryo-probe system installed

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	The DNA binding domai of RTBP1	[U-99% 15N]	1 mM
2	Potassium phosphate	natural abundance	50 mM
3	NaCl	natural abundance	100 mM
4	NaN3	natural abundance	1 mM
5	H2O		90 %
6	D2O		10 %

13 3D HCCH-TOCSY

Instrument

Bruker DRX - 900 MHz cryo-probe system installed

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 Pa, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
7	The DNA binding domai of RTBP1	[U-100% 13C]	1 mM
8	Potassium phosphate	natural abundance	50 mM
9	NaCl	natural abundance	100 mM
10	NaN3	natural abundance	1 mM
11	D2O		100 %

14 3D 1H-13C NOESY

Instrument

Bruker DRX - 900 MHz cryo-probe system installed

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 Pa, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
7	The DNA binding domai of RTBP1	[U-100% 13C]	1 mM
8	Potassium phosphate	natural abundance	50 mM
9	NaCl	natural abundance	100 mM
10	NaN3	natural abundance	1 mM
11	D2O		100 %

15 3D 1H-15N NOESY

Instrument

Bruker DRX - 900 MHz cryo-probe system installed

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	The DNA binding domai of RTBP1	[U-99% 15N]	1 mM
2	Potassium phosphate	natural abundance	50 mM
3	NaCl	natural abundance	100 mM
4	NaN3	natural abundance	1 mM
5	H2O		90 %
6	D2O		10 %

nullKeywords plant, telomere binding protein

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Found 1 Document (0.876 seconds)

BMRB: 11038

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. The DNA binding domain of RTBP1, : 1 : 2 : 21 entities Detail

Experiments performed 15 experiments Detail

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