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BMRB: 11041

2ROP

Solution structure of domains 3 and 4 of human ATP7B

Authors

Banci, L., Bertini, I., Cantini, F., Rosenzweig, A.C., Yatsunyk, L.A.

Assembly

Human ATP7B

Entity

1. Human ATP7B (polymer, Thiol state: all free), 204 monomers, 21327.58 Da Detail

LSSANQNFNN SETLGHQGSH VVTLQLRIDG MHCKSCVLNI EENIGQLLGV QSIQVSLENK TAQVKYDPSC TSPVALQRAI EALPPGNFKV SLPDGAEGSG TDHRSSSSHS PGSPPRNQVQ GTCSTTLIAI AGMTCASCVH SIEGMISQLE GVQQISVSLA EGTATVLYNP AVISPEELRA AIEDMGFEAS VVSESCSTNP LGNH

Formula weight

21327.58 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.2 %, Completeness: 84.3 %, Completeness (bb): 88.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.3 % (1840 of 2182)	84.9 % (953 of 1122)	82.8 % (697 of 842)	87.2 % (190 of 218)
Backbone	88.5 % (1064 of 1202)	90.6 % (375 of 414)	86.6 % (515 of 595)	90.2 % (174 of 193)
Sidechain	80.8 % (943 of 1167)	81.8 % (579 of 708)	80.2 % (348 of 434)	64.0 % (16 of 25)
Aromatic	25.7 % (19 of 74)	32.4 % (12 of 37)	18.9 % (7 of 37)
Methyl	91.5 % (214 of 234)	95.7 % (112 of 117)	87.2 % (102 of 117)

1. The third and fourth copper(I) binding soluble domains

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	The third and fourth copper(I) binding soluble domains	[U-99% 13C; U-99% 15N]	0.1 ~ 0.4 mM
2	Na Pi	natural abundance	20 mM
3	NaCl	natural abundance	150 mM
4	H2O		90 %
5	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	dioxane	carbons	69.3 ppm	null	direct	1.0
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.12 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	dioxane	carbons	69.3 ppm	null	direct	1.0
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.12 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	dioxane	carbons	69.3 ppm	null	direct	1.0
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	dioxane	carbons	69.3 ppm	null	direct	1.0
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.26 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2ROP, Strand ID: A Detail

Release date

2008-06-29

Citation

Metal binding domains 3 and 4 of the Wilson disease protein: solution structure and interaction with the copper(I) chaperone HAH1
Banci, L., Bertini, I., Cantini, F., Rosenzweig, A.C., Yatsunyk, L.A.
Biochemistry (2008), 47, 7423-7429, PubMed 18558714 , DOI 10.1021/bi8004736 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P35670 released on 1994-06-01
Title ATP7B_HUMAN Entity Copper-transporting ATPase 2

Related entities 1. Human ATP7B, : 1 : 2 : 156 entities Detail

Interaction partners 1. Human ATP7B, : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC