BMRB: 11045

Mhr1p-bound ssDNA

Authors

Shibata, T., Ito, Y.

Assembly

5'-D(*DTP*DAP*DCP*DG)-3' 10 structures

Entity

1. DNA (5'-D(*DTP*DAP*DCP*DG)-3') (polymer, Thiol state: not present), 4 monomers, 1253.802 × 10 Da Detail

TACG

2. Mhr1p (polymer, Thiol state: all free), 226 monomers, 26894.37 Da Detail

MKVNHSISRF RPASWFEKTK IIPPQVYIFR NLEYGQVLYS QFPNFSQTQV DKLFVRPNWS NRKPSLRRDI WKCMCVVNLQ NYKQSVHLYQ NLCRLRYLRD VAQRKESDKL RKKDSNGHVW YSGQYRPTYC QEAVADLRES LLKVFENATP AEKQTVPAKK PSIYWEDPWR MGDKDKHWNY DVFNALGLEH KLIQRVGNIA REESVILKEL AKLESHPTEQ TEVSSQ

Total weight

39432.39 Da

Max. entity weight

26894.37 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 4.3 %, Completeness: 2.7 %, Completeness (bb): 7.2 % Detail

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Polymer type: polydeoxyribonucleotide polypeptide(L)

	Total	1H
All	2.7 % (41 of 1532)	2.7 % (41 of 1532)
Backbone	7.2 % (34 of 474)	7.2 % (34 of 474)
Sidechain	0.7 % (7 of 1058)	0.7 % (7 of 1058)
Aromatic	4.1 % (6 of 146)	4.1 % (6 of 146)
Methyl	0.9 % (1 of 108)	0.9 % (1 of 108)

1. DNA (5'-D(*DTP*DAP*DCP*DG)-3')

TACG

2. Mhr1p

MKVNHSISRF RPASWFEKTK IIPPQVYIFR NLEYGQVLYS QFPNFSQTQV DKLFVRPNWS NRKPSLRRDI WKCMCVVNLQ NYKQSVHLYQ NLCRLRYLRD VAQRKESDKL RKKDSNGHVW YSGQYRPTYC QEAVADLRES LLKVFENATP AEKQTVPAKK PSIYWEDPWR MGDKDKHWNY DVFNALGLEH KLIQRVGNIA REESVILKEL AKLESHPTEQ TEVSSQ

Show sample condition

Sample

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.1

#	Name	Isotope labeling	Type	Concentration
1	DNA (5'-D(*DTP*DAP*DCP*DG)-3')	natural abundance	DNA	0.5 mM
2	Mhr1p	natural abundance	protein	0.05 mM
3	MgCl2	natural abundance	salt	6.7 mM
4	NaCl	natural abundance	salt	50 mM
5	D2O		solvent	100 %

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Release date

2009-10-14

Citation

A non-canonical DNA structure enables homologous recombination in various genetic systems
Masuda, T., Ito, Y., Terada, T., Shibata, T., Mikawa, T.
J. Biol. Chem. (2009), 284, 30230-30239, PubMed 19729448 , DOI 10.1074/jbc.M109.043810 , Abstract

Entries sharing articles BMRB: 3 entries Detail

Related entities 2. Mhr1p, : 3 : 3 : 4 entities Detail

Interaction partners 2. Mhr1p, : 14 interactors Detail

Experiments performed 5 experiments Detail