BMRB: 11046

hsRad51-bound ssDNA

Authors

Shibata, T., Ito, Y.

Assembly

5'-D(*DTP*DAP*DCP*DG)-3' 10 structures

Entity

1. DNA (5'-D(*DTP*DAP*DCP*DG)-3') (polymer, Thiol state: not present), 4 monomers, 1253.802 × 10 Da Detail

TACG

2. hsRad51 (polymer, Thiol state: all free), 339 monomers, 36965.76 Da Detail

MAMQMQLEAN ADTSVEEESF GPQPISRLEQ CGINANDVKK LEEAGFHTVE AVAYAPKKEL INIKGISEAK ADKILAEAAK LVPMGFTTAT EFHQRRSEII QITTGSKELD KLLQGGIETG SITEMFGEFR TGKTQICHTL AVTCQLPIDR GGGEGKAMYI DTEGTFRPER LLAVAERYGL SGSDVLDNVA YARAFNTDHQ TQLLYQASAM MVESRYALLI VDSATALYRT DYSGRGELSA RQMHLARFLR MLLRLADEFG VAVVITNQVV AQVDGAAMFA ADPKKPIGGN IIAHASTTRL YLRKGRGETR ICKIYDSPCL PEAEAMFAIN ADGVGDAKD

Total weight

49503.78 Da

Max. entity weight

36965.76 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 4.7 %, Completeness: 3.5 %, Completeness (bb): 6.9 % Detail

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Polymer type: polydeoxyribonucleotide polypeptide(L)

	Total	1H
All	3.5 % (70 of 2008)	3.5 % (70 of 2008)
Backbone	6.9 % (50 of 724)	6.9 % (50 of 724)
Sidechain	1.6 % (20 of 1284)	1.6 % (20 of 1284)
Aromatic	5.0 % (6 of 120)	5.0 % (6 of 120)
Methyl	2.0 % (4 of 204)	2.0 % (4 of 204)

1. DNA (5'-D(*DTP*DAP*DCP*DG)-3')

TACG

2. hsRad51

MAMQMQLEAN ADTSVEEESF GPQPISRLEQ CGINANDVKK LEEAGFHTVE AVAYAPKKEL INIKGISEAK ADKILAEAAK LVPMGFTTAT EFHQRRSEII QITTGSKELD KLLQGGIETG SITEMFGEFR TGKTQICHTL AVTCQLPIDR GGGEGKAMYI DTEGTFRPER LLAVAERYGL SGSDVLDNVA YARAFNTDHQ TQLLYQASAM MVESRYALLI VDSATALYRT DYSGRGELSA RQMHLARFLR MLLRLADEFG VAVVITNQVV AQVDGAAMFA ADPKKPIGGN IIAHASTTRL YLRKGRGETR ICKIYDSPCL PEAEAMFAIN ADGVGDAKD

Show sample condition

Sample

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.1

#	Name	Isotope labeling	Type	Concentration
1	DNA (5'-D(*DTP*DAP*DCP*DG)-3')	natural abundance	DNA	0.5 mM
2	hsRad51	natural abundance	protein	0.05 mM
3	AMP-PNP	natural abundance	additive	10 mM
4	MgCl2	natural abundance	salt	5 mM
5	NaCl	natural abundance	salt	100 mM
6	D2O		protein	100 %

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Release date

2009-10-14

Citation

A non-canonical DNA structure enables homologous recombination in various genetic systems
Masuda, T., Ito, Y., Terada, T., Shibata, T., Mikawa, T.
J. Biol. Chem. (2009), 284, 30230-30239, PubMed 19729448 , DOI 10.1074/jbc.M109.043810 , Abstract

Related entities 2. hsRad51, : 20 : 4 : 2 : 161 entities Detail

Interaction partners 2. hsRad51, : 112 interactors Detail

Experiments performed 5 experiments Detail