BMRB: 11054

Solution structure of a novel insect chemokine isolated from integument

Authors

Kamiya, M., Nakatogawa, S., Oda, Y., Kamijima, T., Aizawa, T., Demura, M., Hayakawa, Y., Kawano, K.

Assembly

insect chemokine

Entity

1. insect chemokine (polymer, Thiol state: all disulfide bound), 32 monomers, 3542.989 Da Detail

SVQILRCPDG MQMLRSGQCV ATTEPPFDPD SY

Formula weight

3542.989 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS7:SG	1:CYS19:SG

Source organism

Mythimna separata

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 84.4 %, Completeness: 75.2 %, Completeness (bb): 91.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	75.2 % (164 of 218)	71.7 % (134 of 187)	96.8 % (30 of 31)
Backbone	91.1 % (82 of 90)	88.7 % (55 of 62)	96.4 % (27 of 28)
Sidechain	64.1 % (82 of 128)	63.2 % (79 of 125)	100.0 % (3 of 3)
Aromatic	55.6 % (5 of 9)	55.6 % (5 of 9)
Methyl	84.6 % (11 of 13)	84.6 % (11 of 13)

1. insect chemokine

SVQILRCPDG MQMLRSGQCV ATTEPPFDPD SY

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 5.1

#	Name	Isotope labeling	Type	Concentration
1	insect chemokine	natural abundance		1.0 ~ 3.0 mM
2	DSS	natural abundance		10 uM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 5.1

#	Name	Isotope labeling	Type	Concentration
3	entity	[U-15N]		1.0 ~ 3.0 mM
4	DSS	natural abundance		10 uM

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 2RPS, Strand ID: A Detail

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Release date

2009-10-13

Citation

A novel peptide mediates aggregation and migration of hemocytes from an insect
Nakatogawa, S., Oda, Y., Kamiya, M., Kamijima, T., Aizawa, T., Clark, K.D., Demura, M., Kawano, K., Strand, M.R., Hayakawa, Y.
Curr. Biol. (2009), 19, 779-785, PubMed 19375321 , DOI 10.1016/j.cub.2009.03.050 , Abstract

Related entities 1. insect chemokine, : 1 : 2 entities Detail

Experiments performed 7 experiments Detail