BMRBj - BMREntryMaster

Found 1 Document (0.941 seconds)

BMRB: 11060

2RPB

Unusual Thermal Disassembly of the SPFH Domain Oligomer from Pyrococcus horikoshii

Authors

Kuwahara, Y., Unzai, S., Nagata, T., Hiroaki, Y., Yokoyama, H., Matsui, I., Ikegami, T., Fujiyoshi, Y., Hiroaki, H.

Assembly

PH0470

Entity

1. PH0470 (polymer, Thiol state: all free), 113 monomers, 12852.60 Da Detail

GSDHVDLREH VIDVPPQEVI CKDNVVVTVD AVVYYQVIDP VKAVYNVSDF LMAIVKLAQT NLRAIIGEME LDETLSGRDI INARLREELD KITDRWGVKI TRVEIQRIDP PKD

Formula weight

12852.6 Da

Source organism

Pyrococcus horikoshii

Exptl. method

solution NMR

Refine. method

distance geometry, torsion angle dynamics, rigid body restrained molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 87.0 %, Completeness (bb): 82.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.0 % (1148 of 1320)	91.9 % (628 of 683)	78.3 % (407 of 520)	96.6 % (113 of 117)
Backbone	82.0 % (548 of 668)	96.4 % (217 of 225)	67.8 % (227 of 335)	96.3 % (104 of 108)
Sidechain	92.9 % (707 of 761)	89.7 % (411 of 458)	97.6 % (287 of 294)	100.0 % (9 of 9)
Aromatic	85.2 % (46 of 54)	70.4 % (19 of 27)	100.0 % (26 of 26)	100.0 % (1 of 1)
Methyl	97.1 % (165 of 170)	98.8 % (84 of 85)	95.3 % (81 of 85)

1. SPFH domain

GSDHVDLREH VIDVPPQEVI CKDNVVVTVD AVVYYQVIDP VKAVYNVSDF LMAIVKLAQT NLRAIIGEME LDETLSGRDI INARLREELD KITDRWGVKI TRVEIQRIDP PKD

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	SPFH domain	[U-100% 13C; U-100% 15N]	0.7 mM
2	HEPES	natural abundance	20 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5

#	Name	Isotope labeling	Concentration
5	SPFH domain	[U-100% 13C]	0.8 mM
6	HEPES	natural abundance	20 mM
7	D2O		100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	dioxane	methylene carbons	69.46 ppm	external	direct	1.0
¹H	TMS	methyl protons	0.0 ppm	external	direct	1.0
¹⁵N	TMS	methyl protons	0.0 ppm	external	indirect	0.1013677

Referencing offset: -0.18 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	dioxane	methylene carbons	69.46 ppm	external	direct	1.0
¹H	TMS	methyl protons	0.0 ppm	external	direct	1.0
¹⁵N	TMS	methyl protons	0.0 ppm	external	indirect	0.1013677

Referencing offset: -0.18 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	dioxane	methylene carbons	69.46 ppm	external	direct	1.0
¹H	TMS	methyl protons	0.0 ppm	external	direct	1.0
¹⁵N	TMS	methyl protons	0.0 ppm	external	indirect	0.1013677

Referencing offset: -0.17 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2RPB, Strand ID: A Detail

Release date

2010-01-10

Citation

Unusual thermal disassembly of the SPFH domain oligomer from Pyrococcus horikoshii
Kuwahara, Y., Unzai, S., Nagata, T., Hiroaki, Y., Yokoyama, H., Matsui, I., Ikegami, T., Fujiyoshi, Y., Hiroaki, H.
Biophys. J. (2009), 97, 2034-2043, PubMed 19804735 , DOI 10.1016/j.bpj.2009.07.034 , Abstract

Related entities 1. PH0470, : 1 : 143 entities Detail

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	SPFH domain	[U-100% 13C; U-100% 15N]	0.7 mM
2	HEPES	natural abundance	20 mM
3	H2O		90 %
4	D2O		10 %

2 3D CBCANH

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	SPFH domain	[U-100% 13C; U-100% 15N]	0.7 mM
2	HEPES	natural abundance	20 mM
3	H2O		90 %
4	D2O		10 %

3 3D CBCA(CO)NH

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	SPFH domain	[U-100% 13C; U-100% 15N]	0.7 mM
2	HEPES	natural abundance	20 mM
3	H2O		90 %
4	D2O		10 %

4 3D HNCO

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	SPFH domain	[U-100% 13C; U-100% 15N]	0.7 mM
2	HEPES	natural abundance	20 mM
3	H2O		90 %
4	D2O		10 %

5 3D HNCA

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	SPFH domain	[U-100% 13C; U-100% 15N]	0.7 mM
2	HEPES	natural abundance	20 mM
3	H2O		90 %
4	D2O		10 %

6 3D HN(CO)CA

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	SPFH domain	[U-100% 13C; U-100% 15N]	0.7 mM
2	HEPES	natural abundance	20 mM
3	H2O		90 %
4	D2O		10 %

7 3D CC(CO)NH

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	SPFH domain	[U-100% 13C; U-100% 15N]	0.7 mM
2	HEPES	natural abundance	20 mM
3	H2O		90 %
4	D2O		10 %

8 3D HCC(CO)NH

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	SPFH domain	[U-100% 13C; U-100% 15N]	0.7 mM
2	HEPES	natural abundance	20 mM
3	H2O		90 %
4	D2O		10 %

9 3D HCCH-TOCSY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5

#	Name	Isotope labeling	Concentration
5	SPFH domain	[U-100% 13C]	0.8 mM
6	HEPES	natural abundance	20 mM
7	D2O		100 %

10 3D 13C-editted NOESY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5

#	Name	Isotope labeling	Concentration
5	SPFH domain	[U-100% 13C]	0.8 mM
6	HEPES	natural abundance	20 mM
7	D2O		100 %

11 3D 15N-editted NOESY

Instrument

Varian INOVA - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	SPFH domain	[U-100% 13C; U-100% 15N]	0.7 mM
2	HEPES	natural abundance	20 mM
3	H2O		90 %
4	D2O		10 %

nullKeywords membrane protein, SPFH domain

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Found 1 Document (0.941 seconds)

BMRB: 11060

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. PH0470, : 1 : 143 entities Detail

Experiments performed 11 experiments Detail

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