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BMRB: 11061

2RPZ

Solution structure of the monomeric form of mouse APOBEC2

Authors

Hayashi, F., Nagata, T., Nagashima, T., Muto, Y., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

APOBEC2

Entity

1. APOBEC2 (polymer, Thiol state: free and other bound), 192 monomers, 21832.43 Da Detail

GSSGSSGRLP VNFFKFQFRN VEYSSGRNKT FLCYVVEVQS KGGQAQATQG YLEDEHAGAH AEEAFFNTIL PAFDPALKYN VTWYVSSSPC AACADRILKT LSKTKNLRLL ILVSRLFMWE EPEVQAALKK LKEAGCKLRI MKPQDFEYIW QNFVEQEEGE SKAFEPWEDI QENFLYYEEK LADILKSGPS SG

2. ZN (non-polymer), 65.409 Da

Total weight

21897.838 Da

Max. entity weight

21832.43 Da

Entity Connection

na 3 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:HIS60:NE2	2:ZN1:ZN
2	na	sing	1:CYS90:SG	2:ZN1:ZN
3	na	sing	1:CYS93:SG	2:ZN1:ZN

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.5 %, Completeness: 96.1 %, Completeness (bb): 95.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.1 % (2213 of 2302)	97.4 % (1170 of 1201)	95.2 % (852 of 895)	92.7 % (191 of 206)
Backbone	95.1 % (1080 of 1136)	96.9 % (376 of 388)	94.5 % (533 of 564)	92.9 % (171 of 184)
Sidechain	97.3 % (1310 of 1346)	97.7 % (794 of 813)	97.1 % (496 of 511)	90.9 % (20 of 22)
Aromatic	95.6 % (239 of 250)	99.2 % (124 of 125)	91.7 % (111 of 121)	100.0 % (4 of 4)
Methyl	97.3 % (179 of 184)	96.7 % (89 of 92)	97.8 % (90 of 92)

1. APOBEC2

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	APOBEC2	[U-13C; U-15N]	protein	1.25 mM
2	TRIS	[U-2H]	buffer	20 mM
3	DTT	[U-2H]	salt	1 mM
4	sodium chloride	natural abundance	salt	100 mM
5	sodium azide	natural abundance	salt	0.02 %
6	ZnCl2	natural abundance	salt	100 uM
7	H2O		solvent	90 %
8	D2O		solvent	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.22 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.22 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2RPZ, Strand ID: A Detail

Release date

2010-03-10

Citation

No title is available
Hayashi, F., Umehara, T., Kigawa, T., Yokoyama, S.

Related entities 1. APOBEC2, : 1 : 2 : 174 entities Detail

Interaction partners 1. APOBEC2, : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC