BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	98.1 % (1251 of 1275)	98.2 % (650 of 662)	98.0 % (491 of 501)	98.2 % (110 of 112)
Backbone	99.1 % (638 of 644)	99.1 % (216 of 218)	99.4 % (320 of 322)	98.1 % (102 of 104)
Sidechain	97.6 % (717 of 735)	97.7 % (434 of 444)	97.2 % (275 of 283)	100.0 % (8 of 8)
Aromatic	91.8 % (90 of 98)	91.8 % (45 of 49)	91.8 % (45 of 49)
Methyl	100.0 % (120 of 120)	100.0 % (60 of 60)	100.0 % (60 of 60)

1. 4.1R FERM alpha-lobe domain

GSHMDPAQLT EDITRYYLCL QLRQDIVAGR LPCSFATLAL LGSYTIQSEL GDYDPELHGV DYVSDFKLAP NQTKELEEKV MELHKSYRSM TPAQADLEFL ENAKKLSMY

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
1	4.1R FERM alpha-lobe domain	natural abundance	protein	0.12 ~ 0.8 mM
2	NaCl		salt	45 mM
3	D2O		solvent	100 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
4	4.1R FERM alpha-lobe domain	[U-98% 15N]	protein	0.12 ~ 0.8 mM
5	NaCl		salt	45 mM
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
8	4.1R FERM alpha-lobe domain	[U-98% 13C; U-98% 15N]	protein	0.12 ~ 0.8 mM
9	NaCl		salt	45 mM
10	H2O		solvent	90 %
11	D2O		solvent	10 %

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
12	4.1R FERM alpha-lobe domain	[U-98% 13C; U-98% 15N]	protein	0.12 ~ 0.8 mM
13	NaCl		salt	45 mM
14	D2O		solvent	100 %

Sample #5

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
15	4.1R FERM alpha-lobe domain	[U-10% 13C; U-98% 15N]	protein	0.48 mM
16	NaCl		salt	45 mM
17	H2O		solvent	90 %
18	D2O		solvent	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.3 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.3 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.34 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2RQ1, Strand ID: A Detail

Release date

2009-04-22

Citation

Solution structure and glycophorin C binding studies of the protein 4.1R FERM alpha-lobe domain
Kusunoki, H., Kohno, T.
Proteins (2009), 76, 255-260, PubMed 19338061 , DOI 10.1002/prot.22405 , Abstract

Related entities 1. 4.1R FERM alpha-lobe domain, : 1 : 4 : 219 entities Detail

Interaction partners 1. 4.1R FERM alpha-lobe domain, : 110 interactors Detail

More details for 110 interactors identified by 11 citations

Experiments performed 21 experiments Detail

1 2D 1H-1H NOESY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
1	4.1R FERM alpha-lobe domain	natural abundance	protein	0.12 ~ 0.8 mM
2	NaCl		salt	45 mM
3	D2O		solvent	100 %

2 3D 1H-15N NOESY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
4	4.1R FERM alpha-lobe domain	[U-98% 15N]	protein	0.12 ~ 0.8 mM
5	NaCl		salt	45 mM
6	H2O		solvent	90 %
7	D2O		solvent	10 %

3 3D 1H-13C NOESY No1

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
8	4.1R FERM alpha-lobe domain	[U-98% 13C; U-98% 15N]	protein	0.12 ~ 0.8 mM
9	NaCl		salt	45 mM
10	H2O		solvent	90 %
11	D2O		solvent	10 %

4 3D 1H-13C NOESY No2

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
12	4.1R FERM alpha-lobe domain	[U-98% 13C; U-98% 15N]	protein	0.12 ~ 0.8 mM
13	NaCl		salt	45 mM
14	D2O		solvent	100 %

5 2D 1H-15N HSQC No1

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
4	4.1R FERM alpha-lobe domain	[U-98% 15N]	protein	0.12 ~ 0.8 mM
5	NaCl		salt	45 mM
6	H2O		solvent	90 %
7	D2O		solvent	10 %

6 2D 1H-15N HSQC No2

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
8	4.1R FERM alpha-lobe domain	[U-98% 13C; U-98% 15N]	protein	0.12 ~ 0.8 mM
9	NaCl		salt	45 mM
10	H2O		solvent	90 %
11	D2O		solvent	10 %

7 2D 1H-13C HSQC No1

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
8	4.1R FERM alpha-lobe domain	[U-98% 13C; U-98% 15N]	protein	0.12 ~ 0.8 mM
9	NaCl		salt	45 mM
10	H2O		solvent	90 %
11	D2O		solvent	10 %

8 2D 1H-13C HSQC No2

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
15	4.1R FERM alpha-lobe domain	[U-10% 13C; U-98% 15N]	protein	0.48 mM
16	NaCl		salt	45 mM
17	H2O		solvent	90 %
18	D2O		solvent	10 %

9 2D 1H-1H TOCSY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
1	4.1R FERM alpha-lobe domain	natural abundance	protein	0.12 ~ 0.8 mM
2	NaCl		salt	45 mM
3	D2O		solvent	100 %

10 3D CBCA(CO)NH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
8	4.1R FERM alpha-lobe domain	[U-98% 13C; U-98% 15N]	protein	0.12 ~ 0.8 mM
9	NaCl		salt	45 mM
10	H2O		solvent	90 %
11	D2O		solvent	10 %

11 3D C(CO)NH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
8	4.1R FERM alpha-lobe domain	[U-98% 13C; U-98% 15N]	protein	0.12 ~ 0.8 mM
9	NaCl		salt	45 mM
10	H2O		solvent	90 %
11	D2O		solvent	10 %

12 3D HNCO

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
8	4.1R FERM alpha-lobe domain	[U-98% 13C; U-98% 15N]	protein	0.12 ~ 0.8 mM
9	NaCl		salt	45 mM
10	H2O		solvent	90 %
11	D2O		solvent	10 %

13 3D HNCA

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
8	4.1R FERM alpha-lobe domain	[U-98% 13C; U-98% 15N]	protein	0.12 ~ 0.8 mM
9	NaCl		salt	45 mM
10	H2O		solvent	90 %
11	D2O		solvent	10 %

14 3D HNCACB

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
8	4.1R FERM alpha-lobe domain	[U-98% 13C; U-98% 15N]	protein	0.12 ~ 0.8 mM
9	NaCl		salt	45 mM
10	H2O		solvent	90 %
11	D2O		solvent	10 %

15 3D H(CCO)NH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
8	4.1R FERM alpha-lobe domain	[U-98% 13C; U-98% 15N]	protein	0.12 ~ 0.8 mM
9	NaCl		salt	45 mM
10	H2O		solvent	90 %
11	D2O		solvent	10 %

16 3D HCCH-TOCSY No1

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
8	4.1R FERM alpha-lobe domain	[U-98% 13C; U-98% 15N]	protein	0.12 ~ 0.8 mM
9	NaCl		salt	45 mM
10	H2O		solvent	90 %
11	D2O		solvent	10 %

17 3D HCCH-TOCSY No2

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
12	4.1R FERM alpha-lobe domain	[U-98% 13C; U-98% 15N]	protein	0.12 ~ 0.8 mM
13	NaCl		salt	45 mM
14	D2O		solvent	100 %

18 3D HNHA

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
8	4.1R FERM alpha-lobe domain	[U-98% 13C; U-98% 15N]	protein	0.12 ~ 0.8 mM
9	NaCl		salt	45 mM
10	H2O		solvent	90 %
11	D2O		solvent	10 %

19 3D HNHB

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
8	4.1R FERM alpha-lobe domain	[U-98% 13C; U-98% 15N]	protein	0.12 ~ 0.8 mM
9	NaCl		salt	45 mM
10	H2O		solvent	90 %
11	D2O		solvent	10 %

20 3D HN(CO)CA

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
8	4.1R FERM alpha-lobe domain	[U-98% 13C; U-98% 15N]	protein	0.12 ~ 0.8 mM
9	NaCl		salt	45 mM
10	H2O		solvent	90 %
11	D2O		solvent	10 %

21 3D HN(CA)CO

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
8	4.1R FERM alpha-lobe domain	[U-98% 13C; U-98% 15N]	protein	0.12 ~ 0.8 mM
9	NaCl		salt	45 mM
10	H2O		solvent	90 %
11	D2O		solvent	10 %

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Found 1 Document (1.602 seconds)

BMRB: 11065

Sample #1

Sample #2

Sample #3

Sample #4

Sample #5

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. 4.1R FERM alpha-lobe domain, : 1 : 4 : 219 entities Detail

Interaction partners 1. 4.1R FERM alpha-lobe domain, : 110 interactors Detail

Experiments performed 21 experiments Detail

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