BMRBj - BMREntryMaster

	Total	¹H
All	95.9 % (141 of 147)	95.9 % (141 of 147)
Backbone	98.4 % (60 of 61)	98.4 % (60 of 61)
Sidechain	94.2 % (81 of 86)	94.2 % (81 of 86)
Aromatic	92.9 % (13 of 14)	92.9 % (13 of 14)
Methyl	93.1 % (27 of 29)	93.1 % (27 of 29)

1. A chain

PAIYIGATVG PSVWAYLVAL VGAAAVTAAN

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 3.5

#	Name	Isotope labeling	Type	Concentration
1	A chain	natural abundance	protein	0.3 ~ 0.35 mM
2	SDS	[U-99% 2H]	lipid	100 mM
3	acetic acid	[U-99% 2H]	buffer	50 mM
4	sodium chloride	natural abundance	salt	20 mM
5	H2O		solvent	95 %
6	D2O		solvent	5 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 3.5

#	Name	Isotope labeling	Type	Concentration
7	A chain	natural abundance	protein	0.3 ~ 0.35 mM
8	SDS	[U-99% 2H]	lipid	100 mM
9	acetic acid	[U-99% 2H]	buffer	50 mM
10	sodium chloride	natural abundance	salt	20 mM
11	D2O		solvent	100 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2RQ2, Strand ID: A Detail

Release date

2009-07-13

Citation

A novel beta-defensin structure: big defensin changes its N-terminal structure to associate with the target membrane
Kouno, T., Mizuguchi, M., Aizawa, T., Shinoda, H., Demura, M., Kawabata, S., Kawano, K.
Biochemistry (2009), 48, 7629-7635, PubMed 19588912 , DOI 10.1021/bi900756y , Abstract

Related entities 1. big-N, : 1 : 2 entities Detail

Experiments performed 6 experiments Detail

1 2D 1H-1H COSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 3.5

#	Name	Isotope labeling	Type	Concentration
1	A chain	natural abundance	protein	0.3 ~ 0.35 mM
2	SDS	[U-99% 2H]	lipid	100 mM
3	acetic acid	[U-99% 2H]	buffer	50 mM
4	sodium chloride	natural abundance	salt	20 mM
5	H2O		solvent	95 %
6	D2O		solvent	5 %

2 2D 1H-1H TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 3.5

#	Name	Isotope labeling	Type	Concentration
1	A chain	natural abundance	protein	0.3 ~ 0.35 mM
2	SDS	[U-99% 2H]	lipid	100 mM
3	acetic acid	[U-99% 2H]	buffer	50 mM
4	sodium chloride	natural abundance	salt	20 mM
5	H2O		solvent	95 %
6	D2O		solvent	5 %

3 2D 1H-1H NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 3.5

#	Name	Isotope labeling	Type	Concentration
1	A chain	natural abundance	protein	0.3 ~ 0.35 mM
2	SDS	[U-99% 2H]	lipid	100 mM
3	acetic acid	[U-99% 2H]	buffer	50 mM
4	sodium chloride	natural abundance	salt	20 mM
5	H2O		solvent	95 %
6	D2O		solvent	5 %

4 2D 1H-1H COSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 3.5

#	Name	Isotope labeling	Type	Concentration
7	A chain	natural abundance	protein	0.3 ~ 0.35 mM
8	SDS	[U-99% 2H]	lipid	100 mM
9	acetic acid	[U-99% 2H]	buffer	50 mM
10	sodium chloride	natural abundance	salt	20 mM
11	D2O		solvent	100 %

5 2D 1H-1H TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 3.5

#	Name	Isotope labeling	Type	Concentration
7	A chain	natural abundance	protein	0.3 ~ 0.35 mM
8	SDS	[U-99% 2H]	lipid	100 mM
9	acetic acid	[U-99% 2H]	buffer	50 mM
10	sodium chloride	natural abundance	salt	20 mM
11	D2O		solvent	100 %

6 2D 1H-1H NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 3.5

#	Name	Isotope labeling	Type	Concentration
7	A chain	natural abundance	protein	0.3 ~ 0.35 mM
8	SDS	[U-99% 2H]	lipid	100 mM
9	acetic acid	[U-99% 2H]	buffer	50 mM
10	sodium chloride	natural abundance	salt	20 mM
11	D2O		solvent	100 %

nullKeywords antimicrobial peptide

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Found 1 Document (0.778 seconds)

BMRB: 11072

Sample #1

Sample #2

Related entities 1. big-N, : 1 : 2 entities Detail

Experiments performed 6 experiments Detail

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