BMRB: 11077

Solution structure of the E. coli ribosome hibernation promoting factor HPF

Authors

Sato, A., Mishima, M.

Assembly

HPF

Entity

1. HPF (polymer, Thiol state: not present), 95 monomers, 10750.11 Da Detail

MQLNITGNNV EITEALREFV TAKFAKLEQY FDRINQVYVV LKVEKVTHTS DATLHVNGGE IHASAEGQDM YAAIDGLIDK LARQLTKHKD KLKQH

Formula weight

10750.11 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 83.0 %, Completeness (bb): 84.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	83.0 % (930 of 1120)	88.4 % (512 of 579)	74.5 % (324 of 435)	88.7 % (94 of 106)
Backbone	84.2 % (480 of 570)	97.9 % (191 of 195)	69.6 % (195 of 280)	98.9 % (94 of 95)
Sidechain	84.4 % (540 of 640)	83.9 % (322 of 384)	89.0 % (218 of 245)	0.0 % (0 of 11)
Aromatic	37.8 % (28 of 74)	37.8 % (14 of 37)	37.8 % (14 of 37)
Methyl	99.2 % (123 of 124)	98.4 % (61 of 62)	100.0 % (62 of 62)

1. HPF

MQLNITGNNV EITEALREFV TAKFAKLEQY FDRINQVYVV LKVEKVTHTS DATLHVNGGE IHASAEGQDM YAAIDGLIDK LARQLTKHKD KLKQH

Show sample condition

Sample #1

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 303 K, pH 6.9

#	Name	Isotope labeling	Type	Concentration
1	HPF	[U-99% 15N]		1 mM
2	potassium phosphate	natural abundance		50 mM
3	potassium chloride	natural abundance		20 mM
4	H2O			93 %
5	D2O			7 %

Sample #2

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 303 K, pH 6.9

#	Name	Isotope labeling	Type	Concentration
6	HPF	[U-99% 13C; U-99% 15N]		1 mM
7	potassium phosphate	natural abundance		50 mM
8	potassium chloride	natural abundance		20 mM
9	H2O			93 %
10	D2O			7 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.9

#	Name	Isotope labeling	Type	Concentration
11	HPF	[U-99% 13C; U-99% 15N]		1 mM
12	potassium phosphate	natural abundance		50 mM
13	potassium chloride	natural abundance		20 mM
14	D2O			100 %

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LACS Plot; CA

Referencing offset: -0.28 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.28 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.09 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2RQL, Strand ID: A Detail

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Release date

2009-10-13

Citation

Solution structure of the E. coli ribosome hibernation promoting factor HPF: Implications for the relationship between structure and function
Sato, A., Watanabe, T., Maki, Y., Ueta, M., Yoshida, H., Ito, Y., Wada, A., Mishima, M.
Biochem. Biophys. Res. Commun. (2009), 389, 580-585, PubMed 19747895 , DOI 10.1016/j.bbrc.2009.09.022 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. HPF, : 1 : 3 : 6 : 86 entities Detail

Experiments performed 13 experiments Detail