BMRB: 11084

Solution structure of the CH domain of human calponin-2

Authors

Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

Calponin-2

Entity

1. Calponin-2 (polymer, Thiol state: all free), 146 monomers, 15793.75 Da Detail

GSSGSSGNRL LSKYDPQKEA ELRTWIEGLT GLSIGPDFQK GLKDGTILCT LMNKLQPGSV PKINRSMQNW HQLENLSNFI KAMVSYGMNP VDLFEANDLF ESGNMTQVQV SLLALAGKAK TKGLQSGVDI GVKYSEKQER SGPSSG

Formula weight

15793.75 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.9 %, Completeness: 95.7 %, Completeness (bb): 94.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.7 % (1603 of 1675)	95.0 % (834 of 878)	96.9 % (617 of 637)	95.0 % (152 of 160)
Backbone	94.6 % (817 of 864)	93.4 % (283 of 303)	95.5 % (402 of 421)	94.3 % (132 of 140)
Sidechain	96.9 % (911 of 940)	95.8 % (551 of 575)	98.6 % (340 of 345)	100.0 % (20 of 20)
Aromatic	97.8 % (90 of 92)	97.8 % (45 of 46)	97.7 % (43 of 44)	100.0 % (2 of 2)
Methyl	100.0 % (144 of 144)	100.0 % (72 of 72)	100.0 % (72 of 72)

1. CH domain

GSSGSSGNRL LSKYDPQKEA ELRTWIEGLT GLSIGPDFQK GLKDGTILCT LMNKLQPGSV PKINRSMQNW HQLENLSNFI KAMVSYGMNP VDLFEANDLF ESGNMTQVQV SLLALAGKAK TKGLQSGVDI GVKYSEKQER SGPSSG

Show sample condition

Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.30mM CH domain [U-15N,13C], 20mM d-Tris-HCl(pH 7.0), 100mM NaCl, 1mM d-DTT, 0.02% NaN3, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CH domain	[U-100% 13C; U-100% 15N]	protein	1.3 mM
2	TRIS	[U-100% 2H]	buffer	20 mM
3	sodium chloride	natural abundance	salt	100 mM
4	DTT	[U-100% 2H]	salt	1 mM
5	sodium azide	natural abundance	salt	0.02 %
6	D2O		solvent	10 %
7	H2O		solvent	90 %

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LACS Plot; CA

Referencing offset: 2.21 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 2.21 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.07 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 1.77 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WYN, Strand ID: A Detail

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Release date

2011-01-03

Citation

Solution structure of the CH domain of human calponin-2
Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. Calponin-2, : 1 : 76 entities Detail

Interaction partners 1. Calponin-2, : 9 interactors Detail

Experiments performed 2 experiments Detail