BMRB: 11085

Solution structure of the CH domain of human NEDD9 interacting protein with calponin homology and LIM domains

Authors

Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

NEDD9 interacting protein with calponin homology and LIM domains

Entity

1. NEDD9 interacting protein with calponin homology and LIM domains (polymer, Thiol state: all free), 116 monomers, 11925.18 Da Detail

GSSGSSGTQE ELLRWCQEQT AGYPGVHVSD LSSSWADGLA LCALVYRLQP GLLEPSELQG LGALEATAWA LKVAENELGI TPVVSAQAVV AGSDPLGLIA YLSHFHSAFK SGPSSG

Formula weight

11925.18 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.7 %, Completeness: 96.0 %, Completeness (bb): 93.9 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.0 % (1189 of 1239)	95.5 % (601 of 629)	97.1 % (476 of 490)	93.3 % (112 of 120)
Backbone	93.9 % (642 of 684)	92.5 % (222 of 240)	95.2 % (318 of 334)	92.7 % (102 of 110)
Sidechain	97.4 % (640 of 657)	96.7 % (376 of 389)	98.4 % (254 of 258)	100.0 % (10 of 10)
Aromatic	100.0 % (92 of 92)	100.0 % (46 of 46)	100.0 % (43 of 43)	100.0 % (3 of 3)
Methyl	100.0 % (144 of 144)	100.0 % (72 of 72)	100.0 % (72 of 72)

1. CH domain

GSSGSSGTQE ELLRWCQEQT AGYPGVHVSD LSSSWADGLA LCALVYRLQP GLLEPSELQG LGALEATAWA LKVAENELGI TPVVSAQAVV AGSDPLGLIA YLSHFHSAFK SGPSSG

Show sample condition

Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.22mM CH domain [U-15N,13C], 20mM d-Tris-HCl, 100mM NaCl, 1mM d-DTT, 0.02% NaN3, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CH domain	[U-100% 13C; U-100% 15N]	protein	1.22 mM
2	TRIS	[U-100% 2H]	buffer	20 mM
3	sodium chloride	natural abundance	salt	100 mM
4	DTT	[U-100% 2H]	salt	1 mM
5	sodium azide	natural abundance	salt	0.02 %
6	D2O		solvent	10 %
7	H2O		solvent	90 %

Hide sample codition

LACS Plot; CA

Referencing offset: 2.1 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: 2.1 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.18 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 2.43 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 1WYL, Strand ID: A Detail

---

Release date

2011-01-03

Citation

Solution structure of the CH domain of human NEDD9 interacting protein with calponin homology and LIM domains
Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. NEDD9 interacting protein with calponin homology and LIM domains, : 1 : 246 entities Detail

Interaction partners 1. NEDD9 interacting protein with calponin homology and LIM domains, : 8 interactors Detail

Experiments performed 2 experiments Detail