BMRB: 11086

Solution structure of the CH domain of human microtubule-associated protein RP/EB family member 3

Authors

Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

Microtubule-associated protein RP/EB family member 3

Entity

1. Microtubule-associated protein RP/EB family member 3 (polymer, Thiol state: all free), 159 monomers, 17890.18 Da Detail

GSSGSSGMAV NVYSTSVTSE NLSRHDMLAW VNDSLHLNYT KIEQLCSGAA YCQFMDMLFP GCVHLRKVKF QAKLEHEYIH NFKVLQAAFK KMGVDKIIPV EKLVKGKFQD NFEFIQWFKK FFDANYDGKD YNPLLARQGQ DVAPPPNPGD QIFSGPSSG

Formula weight

17890.18 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.4 %, Completeness: 96.5 %, Completeness (bb): 96.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.5 % (1830 of 1897)	96.6 % (955 of 989)	96.5 % (711 of 737)	95.9 % (164 of 171)
Backbone	96.3 % (903 of 938)	96.9 % (312 of 322)	96.1 % (447 of 465)	95.4 % (144 of 151)
Sidechain	96.9 % (1072 of 1106)	96.4 % (643 of 667)	97.6 % (409 of 419)	100.0 % (20 of 20)
Aromatic	95.3 % (202 of 212)	95.3 % (101 of 106)	95.2 % (99 of 104)	100.0 % (2 of 2)
Methyl	97.2 % (138 of 142)	97.2 % (69 of 71)	97.2 % (69 of 71)

1. CH domain

GSSGSSGMAV NVYSTSVTSE NLSRHDMLAW VNDSLHLNYT KIEQLCSGAA YCQFMDMLFP GCVHLRKVKF QAKLEHEYIH NFKVLQAAFK KMGVDKIIPV EKLVKGKFQD NFEFIQWFKK FFDANYDGKD YNPLLARQGQ DVAPPPNPGD QIFSGPSSG

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Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.28mM CH domain [U-15N,13C], 20mM d-Tris-HCl(pH 7.0), 100mM NaCl, 1mM d-DTT, 0.02% NaN3, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CH domain	[U-100% 13C; U-100% 15N]	protein	1.28 mM
2	TRIS	[U-100% 2H]	buffer	20 mM
3	sodium chloride	natural abundance	salt	100 mM
4	DTT	[U-100% 2H]	salt	1 mM
5	sodium azide	natural abundance	salt	0.02 %
6	D2O		solvent	10 %
7	H2O		solvent	90 %

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LACS Plot; CA

Referencing offset: 2.42 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 2.42 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.15 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 2.16 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WYO, Strand ID: A Detail

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Release date

2011-01-03

Citation

Solution structure of the CH domain of human microtubule-associated protein RP/EB family member 3
Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. Microtubule-associated protein RP/EB family member 3, : 1 : 1 : 50 entities Detail

Interaction partners 1. Microtubule-associated protein RP/EB family member 3, : 1 interactors Detail

Experiments performed 2 experiments Detail