BMRB: 11087

Solution structure of the PH domain of Kindlin-3 from human

Authors

Harada, T., Li, H., Sato, M., Koshiba, S., Watanabe, S., Kigawa, T., Yokoyama, S.

Assembly

Unc-112-related protein 2

Entity

1. Unc-112-related protein 2 (polymer, Thiol state: all free), 137 monomers, 15460.53 Da Detail

GSSGSSGIPE LKDHLRIFRP RKLTLKGYRQ HWVVFKETTL SYYKSQDEAP GDPIQQLNLK GCEVVPDVNV SGQKFCIKLL VPSPEGMSEI YLRCQDEQQY ARWMAGCRLA SKGRTMADSS YTSEVQAILA FLSLQRT

Formula weight

15460.53 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.1 %, Completeness: 96.0 %, Completeness (bb): 94.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.0 % (1551 of 1616)	95.4 % (809 of 848)	97.1 % (606 of 624)	94.4 % (136 of 144)
Backbone	94.9 % (767 of 808)	93.5 % (259 of 277)	96.3 % (386 of 401)	93.8 % (122 of 130)
Sidechain	97.0 % (907 of 935)	96.3 % (550 of 571)	98.0 % (343 of 350)	100.0 % (14 of 14)
Aromatic	97.5 % (117 of 120)	98.3 % (59 of 60)	96.6 % (56 of 58)	100.0 % (2 of 2)
Methyl	99.3 % (137 of 138)	98.6 % (68 of 69)	100.0 % (69 of 69)

1. PH domain, UNP residues 349-478

GSSGSSGIPE LKDHLRIFRP RKLTLKGYRQ HWVVFKETTL SYYKSQDEAP GDPIQQLNLK GCEVVPDVNV SGQKFCIKLL VPSPEGMSEI YLRCQDEQQY ARWMAGCRLA SKGRTMADSS YTSEVQAILA FLSLQRT

Show sample condition

Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.18mM PH domain [U-15N,13C], 20mM d-Tris-HCl, 100mM NaCl, 1mM d-DTT, 0.02% NaN3, 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	PH domain	[U-13C; U-15N]	protein	1.18 mM
2	TRIS	[U-2H]	buffer	20 mM
3	sodium chloride	natural abundance	salt	100 mM
4	DTT	[U-2H]	salt	1 mM
5	sodium azide	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.0 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.0 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 5 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.16 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2YS3, Strand ID: A Detail

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Release date

2011-01-13

Citation

Solution structure of the PH domain of Kindlin-3 from human
Li, H., Sato, M., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. Unc-112-related protein 2, : 1 : 37 entities Detail

Interaction partners 1. Unc-112-related protein 2, : 11 interactors Detail

Experiments performed 2 experiments Detail