BMRB: 11088

Solution structure of the PH domain of Dynamin-2 from human

Authors

Li, H., Sato, M., Tochio, N., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

Dynamin-2

Entity

1. Dynamin-2 (polymer, Thiol state: all free), 113 monomers, 13059.64 Da Detail

GSSGSSGVIR RGWLTINNIS LMKGGSKEYW FVLTAESLSW YKDEEEKEKK YMLPLDNLKI RDVEKGFMSN KHVFAIFNTE QRNVYKDLRQ IELACDSQED VDSWKASFLR AGV

Formula weight

13059.64 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.7 %, Completeness: 94.0 %, Completeness (bb): 90.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.0 % (1281 of 1363)	94.7 % (675 of 713)	94.1 % (494 of 525)	89.6 % (112 of 125)
Backbone	90.8 % (614 of 676)	91.0 % (212 of 233)	91.5 % (303 of 331)	88.4 % (99 of 112)
Sidechain	96.8 % (767 of 792)	96.5 % (463 of 480)	97.3 % (291 of 299)	100.0 % (13 of 13)
Aromatic	98.5 % (132 of 134)	100.0 % (67 of 67)	96.8 % (61 of 63)	100.0 % (4 of 4)
Methyl	100.0 % (108 of 108)	100.0 % (54 of 54)	100.0 % (54 of 54)

1. PH domain

GSSGSSGVIR RGWLTINNIS LMKGGSKEYW FVLTAESLSW YKDEEEKEKK YMLPLDNLKI RDVEKGFMSN KHVFAIFNTE QRNVYKDLRQ IELACDSQED VDSWKASFLR AGV

Show sample condition

Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.17mM PH domain [U-15N,13C], 20mM d-Tris-HCl, 100mM NaCl, 1mM d-DTT, 0.02% NaN3, 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	PH domain	[U-13C; U-15N]	protein	1.17 mM
2	TRIS	[U-2H]	buffer	20 mM
3	sodium chloride	natural abundance	salt	100 mM
4	DTT	[U-2H]	salt	1 mM
5	sodium azide	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.08 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.08 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.38 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2YS1, Strand ID: A Detail

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Release date

2011-01-18

Citation

Solution structure of the PH domain of Dynamin-2 from human
Li, H., Sato, M., Tochio, N., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. Dynamin-2, : 1 : 7 : 146 entities Detail

Interaction partners 1. Dynamin-2, : 22 interactors Detail

Experiments performed 2 experiments Detail