BMRB: 11089

Solution structure of the PH domain of Pleckstrin homology domain-containing family A member 6 from human

Authors

Li, H., Sato, M., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

Pleckstrin homology domain-containing family A member 6

Entity

1. Pleckstrin homology domain-containing family A member 6 (polymer, Thiol state: all free), 122 monomers, 13709.29 Da Detail

GSSGSSGGKR SHSMKRNPNA PVTKAGWLFK QASSGVKQWN KRWFVLVDRC LFYYKDEKEE SILGSIPLLS FRVAAVQPSD NISRKHTFKA EHAGVRTYFF SAESPEEQEA WIQAMGEAAR VQ

Formula weight

13709.29 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.9 %, Completeness: 93.3 %, Completeness (bb): 90.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.3 % (1341 of 1438)	93.2 % (702 of 753)	94.0 % (521 of 554)	90.1 % (118 of 131)
Backbone	90.7 % (655 of 722)	90.7 % (225 of 248)	91.3 % (326 of 357)	88.9 % (104 of 117)
Sidechain	94.8 % (786 of 829)	93.9 % (474 of 505)	96.1 % (298 of 310)	100.0 % (14 of 14)
Aromatic	97.4 % (150 of 154)	100.0 % (77 of 77)	94.5 % (69 of 73)	100.0 % (4 of 4)
Methyl	98.0 % (100 of 102)	98.0 % (50 of 51)	98.0 % (50 of 51)

1. PH domain

GSSGSSGGKR SHSMKRNPNA PVTKAGWLFK QASSGVKQWN KRWFVLVDRC LFYYKDEKEE SILGSIPLLS FRVAAVQPSD NISRKHTFKA EHAGVRTYFF SAESPEEQEA WIQAMGEAAR VQ

Show sample condition

Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.21mM PH domain [U-15N,13C], 20mM d-Tris-HCl, 100mM NaCl, 1mM d-DTT, 0.02% NaN3, 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	PH domain	[U-13C; U-15N]	protein	1.21 mM
2	TRIS	[U-2H]	buffer	20 mM
3	sodium chloride	natural abundance	salt	100 mM
4	DTT	[U-2H]	salt	1 mM
5	sodium azide	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.29 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.29 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.32 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2YRY, Strand ID: A Detail

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Release date

2011-01-18

Citation

Solution structure of the PH domain of Pleckstrin homology domain-containing family A member 6 from human
Li, H., Sato, M., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. Pleckstrin homology domain-containing family A member 6, : 1 : 2 : 206 entities Detail

Interaction partners 1. Pleckstrin homology domain-containing family A member 6, : 3 interactors Detail

Experiments performed 2 experiments Detail