BMRB: 11092

Solution structure of the chromo domain of Mortality factor 4-like protein 1 from human

Authors

Li, H., Sato, M., Tochio, N., Tomizawa, T., Koshiba, S., Harada, T., Watanabe, S., Kigawa, T., Yokoyama, S.

Assembly

Mortality factor 4-like protein 1

Entity

1. Mortality factor 4-like protein 1 (polymer, Thiol state: all free), 100 monomers, 11504.94 Da Detail

GSSGSSGMAP KQDPKPKFQE GERVLCFHGP LLYEAKCVKV AIKDKQVKYF IHYSGWNKNW DEWVPESRVL KYVDTNLQKQ RELQKANQEQ YAEGKMRGAA

Formula weight

11504.94 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.0 %, Completeness: 93.2 %, Completeness (bb): 90.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.2 % (1137 of 1220)	92.3 % (598 of 648)	95.5 % (441 of 462)	89.1 % (98 of 110)
Backbone	90.2 % (532 of 590)	87.2 % (177 of 203)	93.2 % (272 of 292)	87.4 % (83 of 95)
Sidechain	95.8 % (692 of 722)	94.6 % (421 of 445)	98.1 % (257 of 262)	93.3 % (14 of 15)
Aromatic	99.1 % (113 of 114)	100.0 % (57 of 57)	98.1 % (53 of 54)	100.0 % (3 of 3)
Methyl	100.0 % (76 of 76)	100.0 % (38 of 38)	100.0 % (38 of 38)

1. Chromo domain

GSSGSSGMAP KQDPKPKFQE GERVLCFHGP LLYEAKCVKV AIKDKQVKYF IHYSGWNKNW DEWVPESRVL KYVDTNLQKQ RELQKANQEQ YAEGKMRGAA

Show sample condition

Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.13mM Chromo domain [U-15N, 13C], 20mM d-Tris-HCl(pH7.0), 100mM NaCl, 1mM d-DTT, 0.02% NaN3, 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Chromo domain	[U-13C; U-15N]	protein	1.13 mM
2	TRIS	[U-2H]	buffer	20 mM
3	sodium chloride	natural abundance	salt	100 mM
4	DTT	[U-2H]	salt	1 mM
5	sodium azide	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.16 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.16 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.1 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EFI, Strand ID: A Detail

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Release date

2011-01-18

Citation

Solution structure of the chromo domain of Mortality factor 4-like protein 1 from human
Li, H., Sato, M., Tochio, N., Tomizawa, T., Koshiba, S., Harada, T., Watanabe, S., Kigawa, T., Yokoyama, S.

Related entities 1. Mortality factor 4-like protein 1, : 1 : 3 : 19 entities Detail

Interaction partners 1. Mortality factor 4-like protein 1, : 7 interactors Detail

Experiments performed 2 experiments Detail