BMRB: 11095

Solution structure of the chimera of the PTB domain of SNT-2 and 19-residue peptide (aa 1571-1589) of hALK

Authors

Li, H., Koshiba, S., Tomizawa, T., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

Fibroblast growth factor receptor substrate 3 and ALK tyrosine kinase receptor

Entity

1. Fibroblast growth factor receptor substrate 3 and ALK tyrosine kinase receptor (polymer, Thiol state: all free), 153 monomers, 16935.66 Da Detail

GSSGSSGLNR DSVPDNHPTK FKVTNVDDEG VELGSGVMEL TQSELVLHLH RREAVRWPYL CLRRYGYDSN LFSFESGRRC QTGQGIFAFK CSRAEEIFNL LQDLMQCNSI NVMEEPVIIT SGSSGSSGSS GSSGLFRLRH FPCGNVNYGY QQQ

Formula weight

16935.66 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.2 %, Completeness: 90.3 %, Completeness (bb): 86.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.3 % (1560 of 1728)	90.3 % (813 of 900)	90.9 % (602 of 662)	87.3 % (145 of 166)
Backbone	86.3 % (784 of 908)	84.9 % (270 of 318)	87.6 % (387 of 442)	85.8 % (127 of 148)
Sidechain	94.1 % (900 of 956)	93.3 % (543 of 582)	95.2 % (339 of 356)	100.0 % (18 of 18)
Aromatic	98.6 % (146 of 148)	100.0 % (74 of 74)	97.3 % (71 of 73)	100.0 % (1 of 1)
Methyl	97.0 % (128 of 132)	97.0 % (64 of 66)	97.0 % (64 of 66)

1. PTB domain and 19-residue peptide

GSSGSSGLNR DSVPDNHPTK FKVTNVDDEG VELGSGVMEL TQSELVLHLH RREAVRWPYL CLRRYGYDSN LFSFESGRRC QTGQGIFAFK CSRAEEIFNL LQDLMQCNSI NVMEEPVIIT SGSSGSSGSS GSSGLFRLRH FPCGNVNYGY QQQ

Show sample condition

Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.12mM chimera sample [U-15N, 13C], 20mM d-Tris-HCl, 100mM NaCl, 1mM d-DTT, 0.02% NaN3, 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	PTB domain and 19-residue peptide	[U-13C; U-15N]	protein	1.12 mM
2	TRIS	[U-2H]	buffer	20 mM
3	sodium chloride	natural abundance	salt	100 mM
4	DTT	[U-2H]	salt	1 mM
5	sodium azide	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.21 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.21 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.29 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2YT2, Strand ID: A Detail

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Release date

2011-02-13

Citation

Solution structure of the chimera of the PTB domain of SNT-2 and 19-residue peptide (aa 1571-1589) of hALK
Li, H., Koshiba, S., Tomizawa, T., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. Fibroblast growth factor receptor substrate 3 and ALK tyrosine kinase receptor, : 1 : 1 : 3 entities Detail

Experiments performed 2 experiments Detail