BMRB: 11105

The solution structure of the thioredoxin domain of human Thioredoxin-related transmembrane protein 2

Authors

Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Thioredoxin-related transmembrane protein 2

Entity

1. Thioredoxin-related transmembrane protein 2 (polymer, Thiol state: all free), 137 monomers, 15511.18 Da Detail

GSSGSSGYIK YFNDKTIDEE LERDKRVTWI VEFFANWSND CQSFAPIYAD LSLKYNCTGL NFGKVDVGRY TDVSTRYKVS TSPLTKQLPT LILFQGGKEA MRRPQIDKKG RAVSWTFSEE NVIREFNLNE LSGPSSG

Formula weight

15511.18 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.4 %, Completeness: 94.7 %, Completeness (bb): 93.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.7 % (1529 of 1614)	93.6 % (787 of 841)	96.6 % (605 of 626)	93.2 % (137 of 147)
Backbone	93.8 % (762 of 812)	92.5 % (259 of 280)	95.0 % (380 of 400)	93.2 % (123 of 132)
Sidechain	95.8 % (889 of 928)	94.1 % (528 of 561)	98.6 % (347 of 352)	93.3 % (14 of 15)
Aromatic	98.8 % (162 of 164)	100.0 % (82 of 82)	98.7 % (78 of 79)	66.7 % (2 of 3)
Methyl	98.4 % (126 of 128)	96.9 % (62 of 64)	100.0 % (64 of 64)

1. Thioredoxin domain

GSSGSSGYIK YFNDKTIDEE LERDKRVTWI VEFFANWSND CQSFAPIYAD LSLKYNCTGL NFGKVDVGRY TDVSTRYKVS TSPLTKQLPT LILFQGGKEA MRRPQIDKKG RAVSWTFSEE NVIREFNLNE LSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 0.4mM Thioredoxin domain [U-15N,13C]; 20mM d-Tris HCl; 100mM NaCl; 1mM d-DTT; 0.02% NaN3; 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Thioredoxin domain	[U-13C; U-15N]	protein	0.4 mM
2	d-Tris HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.01 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.01 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.04 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DJ0, Strand ID: A Detail

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Release date

2011-02-17

Citation

The solution structure of the thioredoxin domain of human Thioredoxin-related transmembrane protein 2
Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Thioredoxin-related transmembrane protein 2, : 1 : 2 : 40 entities Detail

Interaction partners 1. Thioredoxin-related transmembrane protein 2, : 8 interactors Detail

Experiments performed 2 experiments Detail