BMRB: 11106

The solution structure of the FN3 domain of human Midline 2 protein

Authors

Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

midline 2 isoform 2

Entity

1. midline 2 isoform 2 (polymer, Thiol state: all free), 127 monomers, 13948.18 Da Detail

GSSGSSGEGL DYLTAPNPPS IREELCTASH DTITVHWISD DEFSISSYEL QYTIFTGQAN FISLYNSVDS WMIVPNIKQN HYTVHGLQSG TRYIFIVKAI NQAGSRNSEP TRLKTNSQPF KSGPSSG

Formula weight

13948.18 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.1 %, Completeness: 95.1 %, Completeness (bb): 94.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.1 % (1367 of 1437)	94.7 % (702 of 741)	96.2 % (539 of 560)	92.6 % (126 of 136)
Backbone	94.5 % (707 of 748)	93.4 % (240 of 257)	95.7 % (355 of 371)	93.3 % (112 of 120)
Sidechain	94.8 % (764 of 806)	94.4 % (457 of 484)	95.8 % (293 of 306)	87.5 % (14 of 16)
Aromatic	91.3 % (126 of 138)	94.2 % (65 of 69)	88.1 % (59 of 67)	100.0 % (2 of 2)
Methyl	96.7 % (118 of 122)	96.7 % (59 of 61)	96.7 % (59 of 61)

1. fn3 domain

GSSGSSGEGL DYLTAPNPPS IREELCTASH DTITVHWISD DEFSISSYEL QYTIFTGQAN FISLYNSVDS WMIVPNIKQN HYTVHGLQSG TRYIFIVKAI NQAGSRNSEP TRLKTNSQPF KSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.2mM fn3 domain [U-15N, 13C]; 20mM d-Tris-HCl; 100mM NaCl; 1mM d-DTT; 0.02% NaN3; 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	fn3 domain	[U-13C; U-15N]	protein	1.2 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.13 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.13 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.4 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DMK, Strand ID: A Detail

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Release date

2011-02-17

Citation

The solution structure of the FN3 domain of human Midline 2 protein
Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. midline 2 isoform 2, : 1 : 3 : 127 entities Detail

Interaction partners 1. midline 2 isoform 2, : 117 interactors Detail

Experiments performed 2 experiments Detail