BMRB: 11108

The solution structure of the thioredoxin domain of human Thioredoxin-like protein 2

Authors

Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Thioredoxin-like protein 2

Entity

1. Thioredoxin-like protein 2 (polymer, Thiol state: all free), 130 monomers, 13771.43 Da Detail

GSSGSSGMAA GAAEAAVAAV EEVGSAGQFE ELLRLKAKSL LVVHFWAPWA PQCAQMNEVM AELAKELPQV SFVKLEAEGV PEVSEKYEIS SVPTFLFFKN SQKIDRLDGA HAPELTKKVQ RHASSGPSSG

Formula weight

13771.43 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.2 %, Completeness: 96.0 %, Completeness (bb): 95.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.0 % (1392 of 1450)	94.9 % (711 of 749)	97.5 % (554 of 568)	95.5 % (127 of 133)
Backbone	95.0 % (728 of 766)	93.2 % (245 of 263)	96.3 % (366 of 380)	95.1 % (117 of 123)
Sidechain	97.1 % (781 of 804)	95.9 % (466 of 486)	99.0 % (305 of 308)	100.0 % (10 of 10)
Aromatic	100.0 % (104 of 104)	100.0 % (52 of 52)	100.0 % (50 of 50)	100.0 % (2 of 2)
Methyl	100.0 % (142 of 142)	100.0 % (71 of 71)	100.0 % (71 of 71)

1. Thioredoxin domain, residues 8-124

GSSGSSGMAA GAAEAAVAAV EEVGSAGQFE ELLRLKAKSL LVVHFWAPWA PQCAQMNEVM AELAKELPQV SFVKLEAEGV PEVSEKYEIS SVPTFLFFKN SQKIDRLDGA HAPELTKKVQ RHASSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.3mM Thioredoxin domain [U-15N,13C]; 20mM d-Tris HCl; 100mM NaCl; 1mM d-DTT; 0.02% NaN3; 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Thioredoxin domain	[U-13C; U-15N]	protein	1.3 mM
2	d-Tris HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.16 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DIY, Strand ID: A Detail

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Release date

2011-02-17

Citation

Solution structure of Escherichia coli glutaredoxin-2 shows similarity to mammalian glutathione-S-transferases
Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.
J. Mol. Biol. (2001), 310, 907-918, PubMed 11453697 , DOI 10.1006/jmbi.2001.4721 , Abstract

Related entities 1. Thioredoxin-like protein 2, : 1 : 3 : 65 entities Detail

Interaction partners 1. Thioredoxin-like protein 2, : 75 interactors Detail

Experiments performed 2 experiments Detail