BMRB: 11112

The solution structure of the fourth fibronectin type III domain of human Neogenin

Authors

Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Neogenin

Entity

1. Neogenin (polymer, Thiol state: not present), 126 monomers, 13604.84 Da Detail

GSSGSSGPAT DWLSAETFES DLDETRVPEV PSSLHVRPLV TSIVVSWTPP ENQNIVVRGY AIGYGIGSPH AQTIKVDYKQ RYYTIENLDP SSHYVITLKA FNNVGEGIPL YESAVTRPHT SGPSSG

Formula weight

13604.84 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.1 %, Completeness: 92.3 %, Completeness (bb): 91.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.3 % (1288 of 1395)	91.3 % (654 of 716)	93.5 % (518 of 554)	92.8 % (116 of 125)
Backbone	91.0 % (668 of 734)	88.9 % (224 of 252)	92.1 % (338 of 367)	92.2 % (106 of 115)
Sidechain	93.8 % (728 of 776)	92.7 % (430 of 464)	95.4 % (288 of 302)	100.0 % (10 of 10)
Aromatic	93.1 % (108 of 116)	94.8 % (55 of 58)	91.1 % (51 of 56)	100.0 % (2 of 2)
Methyl	100.0 % (140 of 140)	100.0 % (70 of 70)	100.0 % (70 of 70)

1. fn3 domain

GSSGSSGPAT DWLSAETFES DLDETRVPEV PSSLHVRPLV TSIVVSWTPP ENQNIVVRGY AIGYGIGSPH AQTIKVDYKQ RYYTIENLDP SSHYVITLKA FNNVGEGIPL YESAVTRPHT SGPSSG

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.5mM FN3 domain [U-15N,13C]; 20mM d-Tris HCl; 100mM NaCl; 1mM d-DTT; 0.02% NaN3; 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	FN3 domain	[U-13C; U-15N]	protein	1.5 mM
2	d-Tris HCl	[U-2H]	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	[U-2H]	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.11 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.11 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.43 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1X5I, Strand ID: A Detail

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Release date

2011-02-17

Citation

The solution structure of the cytokine-binding domain of the common beta-chain of the receptors for granulocyte-macrophage colony-stimulating factor, interleukin-3 and interleukin-5
Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.
J. Mol. Biol. (2000), 297, 989-1001, PubMed 10736232 , DOI 10.1006/jmbi.2000.3610 , Abstract

Related entities 1. Neogenin, : 1 : 83 entities Detail

Interaction partners 1. Neogenin, : 3 interactors Detail

Experiments performed 2 experiments Detail