BMRB: 11113

The solution structure of the thioredoxin-like domain of human Thioredoxin-related transmembrane protein

Authors

Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Thioredoxin domain containing protein 1

Entity

1. Thioredoxin domain containing protein 1 (polymer, Thiol state: all free), 126 monomers, 14394.80 Da Detail

GSSGSSGNVR VITDENWREL LEGDWMIEFY APWCPACQNL QPEWESFAEW GEDLEVNIAK VDVTEQPGLS GRFIINALPT IYHCKDGEFR RYQGPRTKKD FINFISDKEW KSIEPVSSWF SGPSSG

Formula weight

14394.8 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.4 %, Completeness: 96.4 %, Completeness (bb): 95.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.4 % (1436 of 1489)	95.5 % (742 of 777)	97.9 % (565 of 577)	95.6 % (129 of 135)
Backbone	95.8 % (709 of 740)	94.5 % (241 of 255)	97.0 % (356 of 367)	94.9 % (112 of 118)
Sidechain	97.2 % (840 of 864)	96.0 % (501 of 522)	99.1 % (322 of 325)	100.0 % (17 of 17)
Aromatic	100.0 % (182 of 182)	100.0 % (91 of 91)	100.0 % (84 of 84)	100.0 % (7 of 7)
Methyl	99.0 % (101 of 102)	98.0 % (50 of 51)	100.0 % (51 of 51)

1. thioredoxin like domain

GSSGSSGNVR VITDENWREL LEGDWMIEFY APWCPACQNL QPEWESFAEW GEDLEVNIAK VDVTEQPGLS GRFIINALPT IYHCKDGEFR RYQGPRTKKD FINFISDKEW KSIEPVSSWF SGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.3mM thioredoxin like domain [U-15N,13C]; 20mM d-Tris HCl; 100mM NaCl; 1mM d-DTT; 0.02% NaN3; 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	thioredoxin like domain	[U-13C; U-15N]	protein	1.3 mM
2	d-Tris HCl	[U-2H]	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	[U-2H]	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.03 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.03 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.22 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1X5E, Strand ID: A Detail

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Release date

2011-02-17

Citation

The solution structure of the thioredoxin-like domain of human Thioredoxin-related transmembrane protein
Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Thioredoxin domain containing protein 1, : 1 : 190 entities Detail

Interaction partners 1. Thioredoxin domain containing protein 1, : 28 interactors Detail

Experiments performed 2 experiments Detail