BMRB: 11117

The solution structure of the second fibronectin type III domain of mouse Ephrin type-A receptor 1

Authors

Tochio, N., Sasagawa, A., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Ephrin type-A receptor 1

Entity

1. Ephrin type-A receptor 1 (polymer, Thiol state: not present), 107 monomers, 11659.95 Da Detail

GSSGSSGAES LSGLSLKLVK KEPRQLELTW AGSRPRNPGG NLSYELHVLN QDEEWHQMVL EPRVLLTKLQ PDTTYIVRVR TLTPLGPGPF SPDHEFRTSP PSGPSSG

Formula weight

11659.95 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.5 %, Completeness: 92.1 %, Completeness (bb): 89.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.1 % (1124 of 1220)	90.8 % (582 of 641)	94.3 % (448 of 475)	90.4 % (94 of 104)
Backbone	89.3 % (552 of 618)	85.9 % (183 of 213)	91.6 % (284 of 310)	89.5 % (85 of 95)
Sidechain	94.8 % (662 of 698)	93.2 % (399 of 428)	97.3 % (254 of 261)	100.0 % (9 of 9)
Aromatic	97.2 % (70 of 72)	97.2 % (35 of 36)	97.1 % (33 of 34)	100.0 % (2 of 2)
Methyl	100.0 % (106 of 106)	100.0 % (53 of 53)	100.0 % (53 of 53)

1. Fibronectin type-III domain

GSSGSSGAES LSGLSLKLVK KEPRQLELTW AGSRPRNPGG NLSYELHVLN QDEEWHQMVL EPRVLLTKLQ PDTTYIVRVR TLTPLGPGPF SPDHEFRTSP PSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.2mM FN3 domain {U-15N,13C;} 20mM d-Tris {HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	FN3 domain	[U-13C; U-15N]	protein	1.2 mM
2	d-Tris HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.1 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.1 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.92 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1X5A, Strand ID: A Detail

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Release date

2011-03-31

Citation

The solution structure of the second fibronectin type III domain of mouse Ephrin type-A receptor 1
Tochio, N., Sasagawa, A., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Ephrin type-A receptor 1, : 1 : 2 : 55 entities Detail

Interaction partners 1. Ephrin type-A receptor 1, : 2 interactors Detail

Experiments performed 2 experiments Detail