BMRB: 11123

Solution structure of the SANT domain of human SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1

Authors

Tochio, N., Koshiba, S., Satio, K., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1

Entity

1. SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1 (polymer, Thiol state: all free), 79 monomers, 8613.312 Da Detail

GSSGSSGTLA KSKGASAGRE WTEQETLLLL EALEMYKDDW NKVSEHVGSR TQDECILHFL RLPIEDPYLE NSDSGPSSG

Formula weight

8613.312 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.7 %, Completeness: 91.3 %, Completeness (bb): 89.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.3 % (795 of 871)	89.2 % (403 of 452)	94.1 % (317 of 337)	91.5 % (75 of 82)
Backbone	89.7 % (420 of 468)	86.5 % (141 of 163)	91.7 % (210 of 229)	90.8 % (69 of 76)
Sidechain	93.0 % (441 of 474)	90.7 % (262 of 289)	96.6 % (173 of 179)	100.0 % (6 of 6)
Aromatic	100.0 % (58 of 58)	100.0 % (29 of 29)	100.0 % (27 of 27)	100.0 % (2 of 2)
Methyl	100.0 % (72 of 72)	100.0 % (36 of 36)	100.0 % (36 of 36)

1. UNP residues 610-675, SANT domain

GSSGSSGTLA KSKGASAGRE WTEQETLLLL EALEMYKDDW NKVSEHVGSR TQDECILHFL RLPIEDPYLE NSDSGPSSG

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.0mM sample {U-15N,13C;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	UNP residues 610-675, SANT domain	[U-13C; U-15N]	protein	1.0 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.15 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.15 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.49 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2YUS, Strand ID: A Detail

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Release date

2011-03-31

Citation

Solution structure of the SANT domain of human SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1
Tochio, N., Koshiba, S., Satio, K., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1, : 1 : 33 entities Detail

Interaction partners 1. SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1, : 27 interactors Detail

Experiments performed 2 experiments Detail