BMRB: 11127

Solution structure of the 2nd fibronectin type III domain from mouse biregional cell adhesion molecule-related/down-regulated oncogenes (Cdon) binding protein

Authors

Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

biregional cell adhesion molecule-related/down-regulated oncogenes (Cdon)binding protein

Entity

1. biregional cell adhesion molecule-related/down-regulated oncogenes (Cdon)binding protein (polymer, Thiol state: not present), 121 monomers, 12805.21 Da Detail

GSSGSSGSQP DHGRLSPPEA PDRPTISTAS ETSVYVTWIP RGNGGFPIQS FRVEYKKLKK VGDWILATSA IPPSRLSVEI TGLEKGISYK FRVRALNMLG ESEPSAPSRP YVVSGSGPSS G

Formula weight

12805.21 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.3 %, Completeness: 95.5 %, Completeness (bb): 95.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.5 % (1284 of 1344)	95.2 % (669 of 703)	96.8 % (510 of 527)	92.1 % (105 of 114)
Backbone	95.0 % (665 of 700)	94.7 % (230 of 243)	96.0 % (335 of 349)	92.6 % (100 of 108)
Sidechain	96.5 % (725 of 751)	95.4 % (439 of 460)	98.2 % (280 of 285)	100.0 % (6 of 6)
Aromatic	91.1 % (82 of 90)	88.9 % (40 of 45)	93.0 % (40 of 43)	100.0 % (2 of 2)
Methyl	100.0 % (112 of 112)	100.0 % (56 of 56)	100.0 % (56 of 56)

1. fibronectin type III (fn3) domain

GSSGSSGSQP DHGRLSPPEA PDRPTISTAS ETSVYVTWIP RGNGGFPIQS FRVEYKKLKK VGDWILATSA IPPSRLSVEI TGLEKGISYK FRVRALNMLG ESEPSAPSRP YVVSGSGPSS G

Show sample condition

Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.43mM fn3 domain {U-15N,13C;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	fn3 domain	[U-13C; U-15N]	protein	1.43 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 2.42 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 2.42 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.12 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 3.01 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1X4Z, Strand ID: A Detail

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Release date

2011-03-31

Citation

Solution structure of the 2nd fibronectin type III domain from mouse biregional cell adhesion molecule-related/down-regulated oncogenes (Cdon) binding protein
Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. biregional cell adhesion molecule-related/down-regulated oncogenes (Cdon)binding protein, : 1 : 272 entities Detail

Interaction partners 1. biregional cell adhesion molecule-related/down-regulated oncogenes (Cdon)binding protein, : 3 interactors Detail

Experiments performed 2 experiments Detail