BMRB: 11129

Solution structure of the CH domain of human Rho guanine nucleotide exchange factor 6

Authors

Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

Rho guanine nucleotide exchange factor 6

Entity

1. Rho guanine nucleotide exchange factor 6 (polymer, Thiol state: all free), 121 monomers, 12942.60 Da Detail

GSSGSSGEEQ IVTWLISLGV LESPKKTICD PEEFLKSSLK NGVVLCKLIN RLMPGSVEKF CLDPQTEADC INNINDFLKG CATLQVEIFD PDDLYSGVNF SKVLSTLLAV NKATESGPSS G

Formula weight

12942.6 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.5 %, Completeness: 95.9 %, Completeness (bb): 95.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.9 % (1306 of 1362)	95.2 % (670 of 704)	96.8 % (515 of 532)	96.0 % (121 of 126)
Backbone	95.8 % (684 of 714)	95.1 % (234 of 246)	96.3 % (340 of 353)	95.7 % (110 of 115)
Sidechain	96.3 % (731 of 759)	95.2 % (436 of 458)	97.9 % (284 of 290)	100.0 % (11 of 11)
Aromatic	92.9 % (65 of 70)	97.1 % (34 of 35)	88.2 % (30 of 34)	100.0 % (1 of 1)
Methyl	100.0 % (144 of 144)	100.0 % (72 of 72)	100.0 % (72 of 72)

1. CH domain

GSSGSSGEEQ IVTWLISLGV LESPKKTICD PEEFLKSSLK NGVVLCKLIN RLMPGSVEKF CLDPQTEADC INNINDFLKG CATLQVEIFD PDDLYSGVNF SKVLSTLLAV NKATESGPSS G

Show sample condition

Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.32mM CH domain {U-15N,13C;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CH domain	[U-13C; U-15N]	protein	1.32 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 2.32 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 2.32 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.14 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 2.37 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WYR, Strand ID: A Detail

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Release date

2011-03-31

Citation

Solution structure of the CH domain of human Rho guanine nucleotide exchange factor 6
Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. Rho guanine nucleotide exchange factor 6, : 1 : 2 : 80 entities Detail

Interaction partners 1. Rho guanine nucleotide exchange factor 6, : 16 interactors Detail

Experiments performed 2 experiments Detail