BMRB: 11130

Solution structure of two zf-C2H2 domains from human Zinc finger protein 512

Authors

Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

Zinc finger protein 512

Entity

1. zf-C2H2 domains (polymer, Thiol state: all other bound), 96 monomers, 10421.78 Da Detail

GSSGSSGRIR KEPPVYAAGS LEEQWYLEIV DKGSVSCPTC QAVGRKTIEG LKKHMENCKQ EMFTCHHCGK QLRSLAGMKY HVMANHNSLP SGPSSG

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

10552.599 Da

Max. entity weight

10421.78 Da

Entity Connection

na 12 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS106:SG	2:ZN1:ZN
2	na	sing	1:CYS37:SG	2:ZN1:ZN
3	na	sing	1:CYS109:SG	2:ZN1:ZN
4	na	sing	1:CYS40:SG	2:ZN1:ZN
5	na	sing	1:HIS54:NE2	2:ZN1:ZN
6	na	sing	1:CYS129:SG	2:ZN1:ZN
7	na	sing	1:CYS132:SG	2:ZN1:ZN
8	na	sing	1:CYS58:SG	2:ZN1:ZN
9	na	sing	1:CYS65:SG	2:ZN1:ZN
10	na	sing	1:CYS68:SG	2:ZN1:ZN
11	na	sing	1:HIS81:NE2	2:ZN1:ZN
12	na	sing	1:HIS86:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.8 %, Completeness: 94.2 %, Completeness (bb): 90.8 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.2 % (1011 of 1073)	94.9 % (535 of 564)	94.1 % (386 of 410)	90.9 % (90 of 99)
Backbone	90.8 % (514 of 566)	89.9 % (178 of 198)	91.7 % (254 of 277)	90.1 % (82 of 91)
Sidechain	97.3 % (576 of 592)	96.7 % (354 of 366)	98.2 % (214 of 218)	100.0 % (8 of 8)
Aromatic	98.5 % (65 of 66)	100.0 % (33 of 33)	96.9 % (31 of 32)	100.0 % (1 of 1)
Methyl	100.0 % (72 of 72)	100.0 % (36 of 36)	100.0 % (36 of 36)

1. zf-C2H2 domains

GSSGSSGRIR KEPPVYAAGS LEEQWYLEIV DKGSVSCPTC QAVGRKTIEG LKKHMENCKQ EMFTCHHCGK QLRSLAGMKY HVMANHNSLP SGPSSG

Show sample condition

Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.06mM zf-C2H2 domains {U-15N,13C;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.25mM {ZnCl2;} 1mM {IDA;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	zf-C2H2 domains	[U-13C; U-15N]	protein	1.06 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	ZnCl2	natural abundance	salt	0.25 mM
6	IDA	natural abundance	salt	1 mM
7	NaN3	natural abundance	salt	0.02 %
8	H2O		solvent	90 %
9	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: 2.12 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: 2.12 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.15 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 2.46 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 2CTD, Strand ID: A Detail

---

Release date

2011-03-31

Citation

Solution structure of two zf-C2H2 domains from human Zinc finger protein 512
Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. zf-C2H2 domains, : 1 : 4 : 22 entities Detail

Interaction partners 1. zf-C2H2 domains, : 6 interactors Detail

Experiments performed 2 experiments Detail