BMRB: 11132

Solution structure of the HMG box like domain from human hypothetical protein FLJ14904

Authors

Tomizawa, T., Kigawa, T., Sato, M., Koshiba, S., Inoue, M., Kamatari, Y., Yokoyama, S.

Assembly

novel protein

Entity

1. novel protein (polymer, Thiol state: all free), 93 monomers, 10351.47 Da Detail

GSSGSSGMPN RKASRNAYYF FVQEKIPELR RRGLPVARVA DAIPYCSSDW ALLREEEKEK YAEMAREWRA AQGKDPGPSE KQKPVFTSGP SSG

Formula weight

10351.47 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.5 %, Completeness: 91.5 %, Completeness (bb): 90.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.5 % (981 of 1072)	90.5 % (516 of 570)	93.2 % (382 of 410)	90.2 % (83 of 92)
Backbone	90.0 % (488 of 542)	89.8 % (167 of 186)	90.4 % (245 of 271)	89.4 % (76 of 85)
Sidechain	93.0 % (572 of 615)	90.9 % (349 of 384)	96.4 % (216 of 224)	100.0 % (7 of 7)
Aromatic	100.0 % (86 of 86)	100.0 % (43 of 43)	100.0 % (41 of 41)	100.0 % (2 of 2)
Methyl	100.0 % (62 of 62)	100.0 % (31 of 31)	100.0 % (31 of 31)

1. HMG box like domain

GSSGSSGMPN RKASRNAYYF FVQEKIPELR RRGLPVARVA DAIPYCSSDW ALLREEEKEK YAEMAREWRA AQGKDPGPSE KQKPVFTSGP SSG

Show sample condition

Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.06mM HMG box like domain U-15N, {13C;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	HMG box like domain	[U-13C; U-15N]	protein	1.06 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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Calculated from 20 models in PDB: 2CTO, Strand ID: A Detail

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Release date

2011-03-31

Citation

Solution structure of the HMG box like domain from human hypothetical protein FLJ14904
Tomizawa, T., Kigawa, T., Sato, M., Koshiba, S., Inoue, M., Kamatari, Y., Yokoyama, S.

Related entities 1. novel protein, : 1 : 2 : 11 entities Detail

Interaction partners 1. novel protein, : 1 interactors Detail

Experiments performed 2 experiments Detail