BMRB: 11133

Solution structure of the fourth CH domain from human L-plastin

Authors

Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

L-plastin

Entity

1. L-plastin (polymer, Thiol state: all free), 124 monomers, 13366.08 Da Detail

GSSGSSGNDD IIVNWVNETL REAEKSSSIS SFKDPKISTS LPVLDLIDAI QPGSINYDLL KTENLNDDEK LNNAKYAISM ARKIGARVYA LPEDLVEVNP KMVMTVFACL MGKGMKRVSG PSSG

Formula weight

13366.08 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.0 %, Completeness: 92.1 %, Completeness (bb): 92.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.1 % (1284 of 1394)	90.9 % (659 of 725)	93.3 % (504 of 540)	93.8 % (121 of 129)
Backbone	92.6 % (678 of 732)	92.4 % (232 of 251)	92.6 % (336 of 363)	93.2 % (110 of 118)
Sidechain	91.6 % (712 of 777)	90.1 % (427 of 474)	93.8 % (274 of 292)	100.0 % (11 of 11)
Aromatic	100.0 % (56 of 56)	100.0 % (28 of 28)	100.0 % (27 of 27)	100.0 % (1 of 1)
Methyl	99.3 % (139 of 140)	100.0 % (70 of 70)	98.6 % (69 of 70)

1. CH domain

GSSGSSGNDD IIVNWVNETL REAEKSSSIS SFKDPKISTS LPVLDLIDAI QPGSINYDLL KTENLNDDEK LNNAKYAISM ARKIGARVYA LPEDLVEVNP KMVMTVFACL MGKGMKRVSG PSSG

Show sample condition

Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.14mM CH domain {U-15N,13C;} 20mM d-Tris-HCl(pH {7.0);} 200mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CH domain	[U-13C; U-15N]	protein	1.14 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 2.38 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 2.38 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.11 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 2.36 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2D85, Strand ID: A Detail

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Release date

2011-03-31

Citation

Solution structure of the fourth CH domain from human L-plastin
Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. L-plastin, : 1 : 1 : 115 entities Detail

Interaction partners 1. L-plastin, : 10 interactors Detail

Experiments performed 2 experiments Detail