BMRB: 11136

Solution structure of the 12th KH type I domain from human Vigilin

Authors

Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

Vigilin

Entity

1. Vigilin (polymer, Thiol state: not present), 104 monomers, 11158.51 Da Detail

GSSGSSGKEA LEALVPVTIE VEVPFDLHRY VIGQKGSGIR KMMDEFEVNI HVPAPELQSD IIAITGLAAN LDRAKAGLLE RVKELQAEQE DRALRSFKSG PSSG

Formula weight

11158.51 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.1 %, Completeness: 96.1 %, Completeness (bb): 95.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.1 % (1120 of 1165)	95.9 % (582 of 607)	97.4 % (441 of 453)	92.4 % (97 of 105)
Backbone	95.0 % (583 of 614)	94.4 % (201 of 213)	96.4 % (291 of 302)	91.9 % (91 of 99)
Sidechain	97.5 % (629 of 645)	96.7 % (381 of 394)	98.8 % (242 of 245)	100.0 % (6 of 6)
Aromatic	95.7 % (44 of 46)	95.7 % (22 of 23)	95.7 % (22 of 23)
Methyl	100.0 % (124 of 124)	100.0 % (62 of 62)	100.0 % (62 of 62)

1. KH domain

GSSGSSGKEA LEALVPVTIE VEVPFDLHRY VIGQKGSGIR KMMDEFEVNI HVPAPELQSD IIAITGLAAN LDRAKAGLLE RVKELQAEQE DRALRSFKSG PSSG

Show sample condition

Sample

Solvent system 90% H2O,10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.28mM KH domain U-15N, {13C;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	KH domain	[U-13C; U-15N]	protein	1.28 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 2.28 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 2.28 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.08 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 2.29 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2CTK, Strand ID: A Detail

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Release date

2011-03-31

Citation

Solution structure of the 12th KH type I domain from human Vigilin
Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. Vigilin, : 1 : 35 entities Detail

Interaction partners 1. Vigilin, : 64 interactors Detail

Experiments performed 2 experiments Detail