Solution structure of the 1st KH type I domain from human Vigilin
GSSGSSGDIV ARLQTQASAT VAIPKEHHRF VIGKNGEKLQ DLELKTATKI QIPRPDDPSN QIKITGTKEG IEKARHEVLL ISAEQDKRSG PSSG
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 96.5 % (1019 of 1056) | 96.4 % (536 of 556) | 97.3 % (392 of 403) | 93.8 % (91 of 97) |
Backbone | 95.3 % (528 of 554) | 95.3 % (183 of 192) | 96.0 % (262 of 273) | 93.3 % (83 of 89) |
Sidechain | 97.6 % (573 of 587) | 97.0 % (353 of 364) | 98.6 % (212 of 215) | 100.0 % (8 of 8) |
Aromatic | 100.0 % (22 of 22) | 100.0 % (11 of 11) | 100.0 % (11 of 11) | |
Methyl | 100.0 % (102 of 102) | 100.0 % (51 of 51) | 100.0 % (51 of 51) |
1. KH domain
GSSGSSGDIV ARLQTQASAT VAIPKEHHRF VIGKNGEKLQ DLELKTATKI QIPRPDDPSN QIKITGTKEG IEKARHEVLL ISAEQDKRSG PSSGSolvent system 90% H2O, 10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.05mM KH domain {U-15N,13C;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | KH domain | [U-13C; U-15N] | protein | 1.05 mM |
2 | d-Tris-HCl | natural abundance | buffer | 20 mM |
3 | NaCl | natural abundance | salt | 100 mM |
4 | d-DTT | natural abundance | salt | 1 mM |
5 | NaN3 | natural abundance | salt | 0.02 % |
6 | H2O | solvent | 90 % | |
7 | D2O | solvent | 10 % |