BMRB: 11141

Solution structure of the CH domain from human Vav-3 protein

Authors

Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

Vav-3 protein

Entity

1. Vav-3 protein (polymer, Thiol state: all free), 143 monomers, 16043.40 Da Detail

GSSGSSGMEP WKQCAQWLIH CKVLPTNHRV TWDSAQVFDL AQTLRDGVLL CQLLNNLRAH SINLKEINLR PQMSQFLCLK NIRTFLTACC ETFGMRKSEL FEAFDLFDVR DFGKVIETLS RLSRTPIALA TGIRPFPSGP SSG

Formula weight

16043.4 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.5 %, Completeness: 95.2 %, Completeness (bb): 94.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.2 % (1603 of 1684)	94.6 % (829 of 876)	96.2 % (631 of 656)	94.1 % (143 of 152)
Backbone	94.2 % (795 of 844)	93.4 % (269 of 288)	95.0 % (399 of 420)	93.4 % (127 of 136)
Sidechain	96.2 % (937 of 974)	95.2 % (560 of 588)	97.6 % (361 of 370)	100.0 % (16 of 16)
Aromatic	99.3 % (137 of 138)	100.0 % (69 of 69)	98.5 % (65 of 66)	100.0 % (3 of 3)
Methyl	100.0 % (162 of 162)	100.0 % (81 of 81)	100.0 % (81 of 81)

1. CH domain

GSSGSSGMEP WKQCAQWLIH CKVLPTNHRV TWDSAQVFDL AQTLRDGVLL CQLLNNLRAH SINLKEINLR PQMSQFLCLK NIRTFLTACC ETFGMRKSEL FEAFDLFDVR DFGKVIETLS RLSRTPIALA TGIRPFPSGP SSG

Show sample condition

Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.23mM CH domain {U-15N,13C;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CH domain	[U-13C; U-15N]	protein	1.23 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 2.31 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 2.31 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.17 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 2.05 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2D86, Strand ID: A Detail

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Release date

2011-03-31

Citation

Solution structure of the CH domain from human Vav-3 protein
Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. Vav-3 protein, : 1 : 41 entities Detail

Interaction partners 1. Vav-3 protein, : 12 interactors Detail

Experiments performed 2 experiments Detail