BMRB: 11142

Solution structure of the CH domain from human Smoothelin splice isoform L2

Authors

Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

Smoothelin splice isoform L2

Entity

1. Smoothelin splice isoform L2 (polymer, Thiol state: all free), 128 monomers, 14547.14 Da Detail

GSSGSSGIKQ MLLDWCRAKT RGYEHVDIQN FSSSWSDGMA FCALVHNFFP EAFDYGQLSP QNRRQNFEVA FSSAETHADC PQLLDTEDMV RLREPDWKCV YTYIQEFYRC LVQKGLVKTK KSSGPSSG

Formula weight

14547.14 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.0 %, Completeness: 90.8 %, Completeness (bb): 89.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.8 % (1356 of 1493)	90.5 % (704 of 778)	91.5 % (528 of 577)	89.9 % (124 of 138)
Backbone	89.7 % (680 of 758)	90.0 % (234 of 260)	89.9 % (337 of 375)	88.6 % (109 of 123)
Sidechain	91.8 % (784 of 854)	90.7 % (470 of 518)	93.1 % (299 of 321)	100.0 % (15 of 15)
Aromatic	97.6 % (164 of 168)	97.6 % (82 of 84)	97.5 % (79 of 81)	100.0 % (3 of 3)
Methyl	98.0 % (98 of 100)	98.0 % (49 of 50)	98.0 % (49 of 50)

1. CH domain

GSSGSSGIKQ MLLDWCRAKT RGYEHVDIQN FSSSWSDGMA FCALVHNFFP EAFDYGQLSP QNRRQNFEVA FSSAETHADC PQLLDTEDMV RLREPDWKCV YTYIQEFYRC LVQKGLVKTK KSSGPSSG

Show sample condition

Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 0.68mM CH domain {U-15N,13C;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CH domain	[U-13C; U-15N]	protein	0.68 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 2.49 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 2.49 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.2 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 2.66 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2D87, Strand ID: A Detail

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Release date

2011-03-31

Citation

Solution structure of the CH domain from human Smoothelin splice isoform L2
Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. Smoothelin splice isoform L2, : 1 : 116 entities Detail

Experiments performed 2 experiments Detail