BMRB: 11144

Solution structure of the DnaJ domain from human Williams-Beuren syndrome chromosome region 18 protein

Authors

Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Williams-Beuren syndrome chromosome region 18 protein

Entity

1. Williams-Beuren syndrome chromosome region 18 protein (polymer, Thiol state: all free), 99 monomers, 10517.38 Da Detail

GSSGSSGSQG DCSYSRTALY DLLGVPSTAT QAQIKAAYYR QCFLYHPDRN SGSAEAAERF TRISQAYVVL GSATLRRKYD RGLLSDEDLR GPGSGPSSG

Formula weight

10517.38 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.9 %, Completeness: 92.8 %, Completeness (bb): 91.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.8 % (981 of 1057)	91.4 % (500 of 547)	95.6 % (391 of 409)	89.1 % (90 of 101)
Backbone	91.0 % (533 of 586)	88.3 % (182 of 206)	93.7 % (267 of 285)	88.4 % (84 of 95)
Sidechain	95.2 % (531 of 558)	93.3 % (318 of 341)	98.1 % (207 of 211)	100.0 % (6 of 6)
Aromatic	100.0 % (80 of 80)	100.0 % (40 of 40)	100.0 % (40 of 40)
Methyl	98.8 % (85 of 86)	97.7 % (42 of 43)	100.0 % (43 of 43)

1. DnaJ domain

GSSGSSGSQG DCSYSRTALY DLLGVPSTAT QAQIKAAYYR QCFLYHPDRN SGSAEAAERF TRISQAYVVL GSATLRRKYD RGLLSDEDLR GPGSGPSSG

Show sample condition

Sample

Solvent system 90% H2O,10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.14mM Gal-binding lectin U-15N, {13C;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Gal-binding lectin	[U-13C; U-15N]	protein	1.14 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.17 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.17 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.38 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2YUA, Strand ID: A Detail

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Release date

2011-03-31

Citation

Solution structure of the DnaJ domain from human Williams-Beuren syndrome chromosome region 18 protein
Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Williams-Beuren syndrome chromosome region 18 protein, : 1 : 2 : 27 entities Detail

Interaction partners 1. Williams-Beuren syndrome chromosome region 18 protein, : 1 interactors Detail

Experiments performed 2 experiments Detail