BMRB: 11145

Solution structure of the CH domain from human EH domain binding protein 1

Authors

Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

EHBP1 protein

Entity

1. EHBP1 protein (polymer, Thiol state: all free), 119 monomers, 13170.83 Da Detail

GSSGSSGPNA SQSLLVWCKE VTKNYRGVKI TNFTTSWRNG LSFCAILHHF RPDLIDYKSL NPQDIKENNK KAYDGFASIG ISRLLEPSDM VLLAIPDKLT VMTYLYQIRA HFSSGPSSG

Formula weight

13170.83 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.8 %, Completeness: 94.6 %, Completeness (bb): 92.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.6 % (1309 of 1384)	93.9 % (674 of 718)	96.1 % (520 of 541)	92.0 % (115 of 125)
Backbone	92.6 % (650 of 702)	90.5 % (218 of 241)	94.5 % (329 of 348)	91.2 % (103 of 113)
Sidechain	96.6 % (765 of 792)	95.6 % (456 of 477)	98.0 % (297 of 303)	100.0 % (12 of 12)
Aromatic	99.2 % (125 of 126)	100.0 % (63 of 63)	98.4 % (60 of 61)	100.0 % (2 of 2)
Methyl	100.0 % (124 of 124)	100.0 % (62 of 62)	100.0 % (62 of 62)

1. CH domain

GSSGSSGPNA SQSLLVWCKE VTKNYRGVKI TNFTTSWRNG LSFCAILHHF RPDLIDYKSL NPQDIKENNK KAYDGFASIG ISRLLEPSDM VLLAIPDKLT VMTYLYQIRA HFSSGPSSG

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Sample

Solvent system 90% H2O,10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 0.85mM CH domain U-15N, {13C;} 20mM d-Tris-HCl(pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2

#	Name	Isotope labeling	Type	Concentration
1	CH domain	[U-13C; U-15N]	protein	0.85 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 2.4 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 2.4 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.16 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 2.69 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2D89, Strand ID: A Detail

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Release date

2011-03-31

Citation

Solution structure of the CH domain from human EH domain binding protein 1
Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. EHBP1 protein, : 1 : 4 : 256 entities Detail

Interaction partners 1. EHBP1 protein, : 6 interactors Detail

Experiments performed 2 experiments Detail