BMRB: 11148

Solution structure of the TIG domain from Human Nuclear factor of activated T-cells, cytoplasmic 4

Authors

Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

Nuclear factor of activated T-cells, cytoplasmic 4

Entity

1. Nuclear factor of activated T-cells, cytoplasmic 4 (polymer, Thiol state: all free), 114 monomers, 12657.03 Da Detail

GSSGSSGLPQ VEAYSPSACS VRGGEELVLT GSNFLPDSKV VFIERGPDGK LQWEEEATVN RLQSNEVTLT LTVPEYSNKR VSRPVQVYFY VSNGRRKRSP TQSFRFLPVI CKEE

Formula weight

12657.03 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 99.3 %, Completeness (bb): 99.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	99.3 % (1305 of 1314)	99.0 % (681 of 688)	100.0 % (509 of 509)	98.3 % (115 of 117)
Backbone	99.4 % (664 of 668)	99.1 % (227 of 229)	100.0 % (333 of 333)	98.1 % (104 of 106)
Sidechain	99.3 % (746 of 751)	98.9 % (454 of 459)	100.0 % (281 of 281)	100.0 % (11 of 11)
Aromatic	100.0 % (94 of 94)	100.0 % (47 of 47)	100.0 % (46 of 46)	100.0 % (1 of 1)
Methyl	100.0 % (114 of 114)	100.0 % (57 of 57)	100.0 % (57 of 57)

1. TIG domain

GSSGSSGLPQ VEAYSPSACS VRGGEELVLT GSNFLPDSKV VFIERGPDGK LQWEEEATVN RLQSNEVTLT LTVPEYSNKR VSRPVQVYFY VSNGRRKRSP TQSFRFLPVI CKEE

Show sample condition

Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 0.94mM TIG domain {U-15N,13C;} 20mM d-Tris-HCl (pH {7.0);} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	TIG domain	[U-13C; U-15N]	protein	0.94 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 2.41 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 2.41 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 2.99 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2YRP, Strand ID: A Detail

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Release date

2011-03-31

Citation

Solution structure of the TIG domain from Human Nuclear factor of activated T-cells, cytoplasmic 4
Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. Nuclear factor of activated T-cells, cytoplasmic 4, : 1 : 2 : 70 entities Detail

Interaction partners 1. Nuclear factor of activated T-cells, cytoplasmic 4, : 11 interactors Detail

Experiments performed 2 experiments Detail