BMRB: 11153

Solution structure of the SH3 domain of 130 kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein

Authors

Abe, H., Tochio, N., Miyamoto, K., Saito, K., Kigawa, T., Yokoyama, S.

Assembly

130 kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein

Entity

1. 130 kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein (polymer, Thiol state: all free), 76 monomers, 8293.934 Da Detail

GSSGSSGNKV RRVKTIYDCQ ADNDDELTFI EGEVIIVTGE EDQEWWIGHI EGQPERKGVF PVSFVHILSD SGPSSG

Formula weight

8293.934 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.8 %, Completeness: 91.7 %, Completeness (bb): 86.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.7 % (773 of 843)	92.9 % (405 of 436)	91.4 % (299 of 327)	86.3 % (69 of 80)
Backbone	86.9 % (391 of 450)	87.4 % (139 of 159)	87.2 % (190 of 218)	84.9 % (62 of 73)
Sidechain	96.3 % (442 of 459)	96.0 % (266 of 277)	96.6 % (169 of 175)	100.0 % (7 of 7)
Aromatic	100.0 % (70 of 70)	100.0 % (35 of 35)	100.0 % (33 of 33)	100.0 % (2 of 2)
Methyl	100.0 % (72 of 72)	100.0 % (36 of 36)	100.0 % (36 of 36)

1. SH3 domain

GSSGSSGNKV RRVKTIYDCQ ADNDDELTFI EGEVIIVTGE EDQEWWIGHI EGQPERKGVF PVSFVHILSD SGPSSG

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.15mM {Protein;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O, 90% H2O

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain	[U-13C; U-15N]	protein	1.15 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.12 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.12 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.12 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.29 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2ED1, Strand ID: A Detail

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Release date

2011-05-04

Citation

Solution structure of the SH3 domain of 130 kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein
Abe, H., Tochio, N., Miyamoto, K., Saito, K., Kigawa, T., Yokoyama, S.

Related entities 1. 130 kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein, : 1 : 9 : 184 entities Detail

Interaction partners 1. 130 kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein, : 11 interactors Detail

Experiments performed 2 experiments Detail