BMRB: 11154

Solution structure of the SH3 domain of Sorbin and SH3 domain-containing protein 1

Authors

Abe, H., Miyamoto, K., Tochio, N., Saito, K., Kigawa, T., Yokoyama, S.

Assembly

Sorbin and SH3 domain-containing protein 1

Entity

1. Sorbin and SH3 domain-containing protein 1 (polymer, Thiol state: not present), 70 monomers, 7556.229 Da Detail

GSSGSSGGEA IAKFNFNGDT QVEMSFRKGE RITLLRQVDE NWYEGRIPGT SRQGIFPITY VDVISGPSSG

Formula weight

7556.229 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.0 %, Completeness: 89.5 %, Completeness (bb): 86.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	89.5 % (698 of 780)	87.4 % (355 of 406)	93.0 % (279 of 300)	86.5 % (64 of 74)
Backbone	86.5 % (358 of 414)	83.1 % (123 of 148)	89.4 % (178 of 199)	85.1 % (57 of 67)
Sidechain	92.7 % (394 of 425)	89.9 % (232 of 258)	96.9 % (155 of 160)	100.0 % (7 of 7)
Aromatic	100.0 % (68 of 68)	100.0 % (34 of 34)	100.0 % (33 of 33)	100.0 % (1 of 1)
Methyl	100.0 % (60 of 60)	100.0 % (30 of 30)	100.0 % (30 of 30)

1. SH3 domain

GSSGSSGGEA IAKFNFNGDT QVEMSFRKGE RITLLRQVDE NWYEGRIPGT SRQGIFPITY VDVISGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 0.75mM {Protein;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O, 90% H2O

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain	[U-13C; U-15N]	protein	0.75 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.17 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.17 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.55 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2ECZ, Strand ID: A Detail

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Release date

2011-05-04

Citation

Solution structure of the SH3 domain of Sorbin and SH3 domain-containing protein 1
Abe, H., Miyamoto, K., Tochio, N., Saito, K., Kigawa, T., Yokoyama, S.

Related entities 1. Sorbin and SH3 domain-containing protein 1, : 1 : 8 : 197 entities Detail

Interaction partners 1. Sorbin and SH3 domain-containing protein 1, : 29 interactors Detail

Experiments performed 2 experiments Detail