BMRB: 11155

Solution structure of the SH3 domain of Abl interactor 2 (Abelson interactor 2)

Authors

Abe, H., Tochio, N., Miyamoto, K., Saito, K., Kigawa, T., Yokoyama, S.

Assembly

Abl interactor 2

Entity

1. Abl interactor 2 (polymer, Thiol state: not present), 78 monomers, 8427.117 Da Detail

GSSGSSGDPP WAPRSYLEKV VAIYDYTKDK EDELSFQEGA IIYVIKKNDD GWYEGVMNGV TGLFPGNYVE SISGPSSG

Formula weight

8427.117 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.6 %, Completeness: 94.7 %, Completeness (bb): 91.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.7 % (823 of 869)	95.1 % (427 of 449)	95.3 % (325 of 341)	89.9 % (71 of 79)
Backbone	91.5 % (419 of 458)	90.7 % (147 of 162)	92.8 % (207 of 223)	89.0 % (65 of 73)
Sidechain	97.9 % (468 of 478)	97.6 % (280 of 287)	98.4 % (182 of 185)	100.0 % (6 of 6)
Aromatic	100.0 % (92 of 92)	100.0 % (46 of 46)	100.0 % (44 of 44)	100.0 % (2 of 2)
Methyl	100.0 % (66 of 66)	100.0 % (33 of 33)	100.0 % (33 of 33)

1. SH3 domain

GSSGSSGDPP WAPRSYLEKV VAIYDYTKDK EDELSFQEGA IIYVIKKNDD GWYEGVMNGV TGLFPGNYVE SISGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 0.73mM {Protein;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O, 90% H2O

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain	[U-13C; U-15N]	protein	0.73 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.17 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.17 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.74 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2ED0, Strand ID: A Detail

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Release date

2011-05-04

Citation

Solution structure of the SH3 domain of Abl interactor 2 (Abelson interactor 2)
Abe, H., Tochio, N., Miyamoto, K., Saito, K., Kigawa, T., Yokoyama, S.

Related entities 1. Abl interactor 2, : 1 : 4 : 124 entities Detail

Interaction partners 1. Abl interactor 2, : 63 interactors Detail

Experiments performed 2 experiments Detail