BMRB: 11156

Solution structure of the SH3 domain of human Nostrin

Authors

Abe, H., Tochio, N., Miyamoto, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Nostrin

Entity

1. Nostrin (polymer, Thiol state: all free), 79 monomers, 8413.148 Da Detail

GSSGSSGRLC KALYSFQARQ DDELNLEKGD IVIIHEKKEE GWWFGSLNGK KGHFPAAYVE ELPSNAGNTA TKASGPSSG

Formula weight

8413.148 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.2 %, Completeness: 94.5 %, Completeness (bb): 92.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.5 % (829 of 877)	94.7 % (433 of 457)	95.8 % (322 of 336)	88.1 % (74 of 84)
Backbone	92.3 % (432 of 468)	92.2 % (153 of 166)	93.8 % (212 of 226)	88.2 % (67 of 76)
Sidechain	96.9 % (462 of 477)	96.2 % (280 of 291)	98.3 % (175 of 178)	87.5 % (7 of 8)
Aromatic	100.0 % (78 of 78)	100.0 % (39 of 39)	100.0 % (37 of 37)	100.0 % (2 of 2)
Methyl	100.0 % (62 of 62)	100.0 % (31 of 31)	100.0 % (31 of 31)

1. SH3 domain

GSSGSSGRLC KALYSFQARQ DDELNLEKGD IVIIHEKKEE GWWFGSLNGK KGHFPAAYVE ELPSNAGNTA TKASGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 1.1mM {Protein;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% NaN3 {;'90%} H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain	[U-13C; U-15N]	protein	1.1 mM
2	d-Tris-HCl	natural abundance	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	d-DTT	natural abundance	salt	1 mM
5	NaN3	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.07 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.07 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.41 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2YUN, Strand ID: A Detail

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Release date

2011-05-04

Citation

Solution structure of the SH3 domain of human Nostrin
Abe, H., Miyamoto, K., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Nostrin, : 1 : 134 entities Detail

Interaction partners 1. Nostrin, : 14 interactors Detail

Experiments performed 2 experiments Detail